Anti-Transglutaminase 2 antibody [EPR2956] (ab109121)
![Anti-Transglutaminase 2 antibody [EPR2956] (ab109121)](https://www.abcam.com/ps/products/109/ab109121/Images/ab109121-390842-anti-transglutaminase-2-antibody-epr2956-western-blot-wildtype-a549-lysates.jpg "Anti-Transglutaminase 2 antibody [EPR2956] (ab109121)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2956] to Transglutaminase 2
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Transglutaminase 2 antibody [EPR2956]
See all Transglutaminase 2 primary antibodies -
Description
Rabbit monoclonal [EPR2956] to Transglutaminase 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A549, ECV-304 and HUVEC cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2956 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Transglutaminase 2 antibody [EPR2956] (ab109121)All lanes : Anti-Transglutaminase 2 antibody [EPR2956] (ab109121) at 1/1000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : TGM2 Knockout A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 77 kDa
Observed band size: 77 kDaLanes 1-2: Merged signal (red and green). Green - ab109121 observed at 77 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109121 Anti-Transglutaminase 2 antibody [EPR2956] was shown to specifically react with Transglutaminase 2 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267110 (knockout cell lysate ab257087) was used. Wild-type and Transglutaminase 2 knockout samples were subjected to SDS-PAGE. ab109121 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Transglutaminase 2 antibody [EPR2956] (ab109121)](https://www.abcam.com/ps/products/109/ab109121/Images/ab109121-303377-anti-transglutaminase-2-antibody-epr2956-western-blot.jpg)
Western blot - Anti-Transglutaminase 2 antibody [EPR2956] (ab109121)All lanes : Anti-Transglutaminase 2 antibody [EPR2956] (ab109121) at 1/1000 dilution
Lane 1 : Mouse heart tissue lysate
Lane 2 : Rat heart tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 77 kDa
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST.
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![Western blot - Anti-Transglutaminase 2 antibody [EPR2956] (ab109121)](https://www.abcam.com/ps/products/109/ab109121/Images/ab109121-121708-anti-transglutaminase-2-antibody-epr2956-western-blot.jpg)
Western blot - Anti-Transglutaminase 2 antibody [EPR2956] (ab109121)All lanes : Anti-Transglutaminase 2 antibody [EPR2956] (ab109121) at 1/1000 dilution
Lane 1 : ECV-304 cell lysates
Lane 2 : HUVEC cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit at 1/2000 dilution
Predicted band size: 77 kDa
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![Anti-Transglutaminase 2 antibody [EPR2956] (ab109121)](https://www.abcam.com/ps/products/109/ab109121/Images/ab109121-4-benefits-of-recombinant-antibodies.png)
Anti-Transglutaminase 2 antibody [EPR2956] (ab109121)
