Anti-Transglutaminase 2 antibody [EP2957] (ab109200)
![Anti-Transglutaminase 2 antibody [EP2957] (ab109200)](https://www.abcam.com/ps/products/109/ab109200/Images/ab109200-390845-anti-transglutaminase-2-antibody-ep2957-western-blot-wildtype-a549-lysates.jpg "Anti-Transglutaminase 2 antibody [EP2957] (ab109200)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2957] to Transglutaminase 2
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Transglutaminase 2 antibody [EP2957]
See all Transglutaminase 2 primary antibodies -
Description
Rabbit monoclonal [EP2957] to Transglutaminase 2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: U87-MG, A549, HeLa and HUVEC cell lysates. IHC-P: Human kidney tissue. IHC-Fr: Mouse kidney and heart tissue.
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General notes
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP2957 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Transglutaminase 2 antibody [EP2957] (ab109200)All lanes : Anti-Transglutaminase 2 antibody [EP2957] (ab109200) at 1/10000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : TGM2 Knockout A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 77 kDa
Observed band size: 77 kDaLanes 1-2: Merged signal (red and green). Green - ab109200 observed at 77 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109200 Anti-Transglutaminase 2 antibody [EP2957] was shown to specifically react with Transglutaminase 2 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab267110 (knockout cell lysate ab257087) was used. Wild-type and Transglutaminase 2 knockout samples were subjected to SDS-PAGE. ab109200 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Transglutaminase 2 antibody [EP2957] (ab109200)](https://www.abcam.com/ps/products/109/ab109200/Images/ab109200-386636-anti-transglutaminase-2-antibody-ep2957-western-blot-human-lysate.jpg)
Western blot - Anti-Transglutaminase 2 antibody [EP2957] (ab109200)All lanes : Anti-Transglutaminase 2 antibody [EP2957] (ab109200) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TGM2 knockout HeLa cell lysate
Lane 3 : Wild-type A549 cell lysate
Lane 4 : TGM2 knockout A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 77 kDa
Observed band size: 77 kDaLanes 1-4: Merged signal (red and green). Green - ab109200 observed at 77 kDa. Red - loading control ab8245 observed at 37 kDa.
ab109200 Anti-Transglutaminase 2 antibody [EP2957] was shown to specifically react with Transglutaminase 2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265245 (knockout cell lysate ab257085) was used. Wild-type and Transglutaminase 2 knockout samples were subjected to SDS-PAGE. ab109200 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Transglutaminase 2 antibody [EP2957] (ab109200)](https://www.abcam.com/ps/products/109/ab109200/Images/ab109200-349465-anti-transglutaminase-2-antibody-ep2957-western-blot-knock-out-og-a549-tgm2-hela-huvec-humanlysates.jpg)
Western blot - Anti-Transglutaminase 2 antibody [EP2957] (ab109200)All lanes : Anti-Transglutaminase 2 antibody [EP2957] (ab109200) at 1/1000 dilution
Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : TGM2 knockout A549 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HUVEC whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 77 kDaLanes 1 - 4: Merged signal (red and green). Green - ab109200 observed at 77 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109200 was shown to recognize TGM2 in wild-type A549 cells as signal was lost at the expected MW in TGM2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and TGM2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab109200 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Transglutaminase 2 antibody [EP2957] (ab109200)](https://www.abcam.com/ps/products/109/ab109200/Images/ab109200-121826-anti-transglutaminase-2-antibody-ep2957-western-blot.jpg)
Western blot - Anti-Transglutaminase 2 antibody [EP2957] (ab109200)All lanes : Anti-Transglutaminase 2 antibody [EP2957] (ab109200) at 1/10000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 77 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EP2957] (ab109200)](https://www.abcam.com/ps/products/109/ab109200/Images/ab109200-121825-anti-transglutaminase-2-antibody-ep2957-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Transglutaminase 2 antibody [EP2957] (ab109200)ab109200, at 1/100 dilution, staining Transglutaminase 2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-Transglutaminase 2 antibody [EP2957] (ab109200)](https://www.abcam.com/ps/products/109/ab109200/Images/ab109200-7-benefits-of-recombinant-antibodies.png)
Anti-Transglutaminase 2 antibody [EP2957] (ab109200)
