Antibodies, Proteins, Kits and Reagents for Life Science

268 032 ₸ Product size

100 µg 268 032 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Rabbit polyclonal to MMP8
Suitable for: WB
Reacts with: Human
Isotype: IgG

Product name

Anti-MMP8 antibody
See all MMP8 primary antibodies
Description

Rabbit polyclonal to MMP8
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human MMP8 aa 350-450 conjugated to keyhole limpet haemocyanin.
(Peptide available as ab94385)
Positive control
- This antibody gave a positive signal in the following human lysates: HeLa whole cell; bone marrow tissue; skin tissue.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS

Batches of this product that have a concentration
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Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas
- Cancer
- Tumor biomarkers
- Enzymes
- MMPs

Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
Recombinant Protein
- Recombinant Human MMP8 protein (ab158906)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab72897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application	Species
WB	Human
All applications	Mouse

Application	Abreviews	Notes
WB

Notes

Application notes

WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 53 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Function

Can degrade fibrillar type I, II, and III collagens.
Tissue specificity

Neutrophils.
Sequence similarities

Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
Domain

The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localization

Cytoplasmic granule. Secreted > extracellular space > extracellular matrix. Stored in intracellular granules.
Target information above from: UniProt accession P22894 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 4317 Human
- Entrez Gene: 17394 Mouse
- Omim: 120355 Human
- SwissProt: P22894 Human
- SwissProt: O70138 Mouse
- Unigene: 161839 Human
- Unigene: 16415 Mouse
Alternative names
- CLG 1 antibody
- CLG1 antibody
- Collagenase 1 antibody
- Collagenase 1 neutrophil antibody
- HNC antibody
- Matrix metallopeptidase 8 (neutrophil collagenase) antibody
- Matrix metalloprotease 8 antibody
- Matrix metalloproteinase-8 antibody
- MMP 8 antibody
- MMP-8 antibody
- Mmp8 antibody
- MMP8_HUMAN antibody
- Neutrophil collagenase antibody
- PMNL CL antibody
- PMNL collagenase antibody
- PMNL-CL antibody
- PMNLCL antibody
see all

Western blot - Anti-MMP8 antibody (ab72897)

All lanes : Anti-MMP8 antibody (ab72897) at 1 µg/ml

Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Human skin tissue lysate - total protein (ab30166)

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 53 kDa
Observed band size: 55 kDa
why is the actual band size different from the predicted?

Exposure time: 2 minutes

Human Neutrophil collagenase precursor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

Western blot protocols

Click here to view the general protocols

Datasheet

Publishing research using ab72897? Please let us know so that we can cite the reference in this datasheet.

ab72897 has not yet been referenced specifically in any publications.

Western blot - Anti-MMP8 antibody (ab72897)

All lanes : Anti-MMP8 antibody (ab72897) at 1 µg/ml

Lane 1 : Bone Marrow (Human) Tissue Lysate - adult normal tissue
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : Human skin tissue lysate - total protein (ab30166)

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 53 kDa
Observed band size: 55 kDa
why is the actual band size different from the predicted?

Exposure time: 2 minutes

Human Neutrophil collagenase precursor contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.