Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620)
Key features and details
- Rabbit polyclonal to Proteasome Activator Subunit 4/PSME4
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Proteasome Activator Subunit 4/PSME4 antibody
See all Proteasome Activator Subunit 4/PSME4 primary antibodies -
Description
Rabbit polyclonal to Proteasome Activator Subunit 4/PSME4 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Mouse
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Immunogen
Synthetic peptide corresponding to Human Proteasome Activator Subunit 4/PSME4 aa 1620-1634.
Sequence:TKLPKKRKRDPGSVG
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General notes
This product was previously labelled as Proteasome Activator Subunit 4
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620)Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620) at 1/2000 dilution + Mouse testis tissue lysate at 25 µg
Secondary
HRP-conjugated anti-rabbit
Developed using the ECL technique.
Observed band size: 160,200 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-Proteasome Activator Subunit 4/PSME4 antibody (ab5620)ICC/IF image of ab5620 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5620, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
