Human CELF1 (CUG-BP1) knockout HEK-293T cell line (ab266086)
Properties
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Number of cells
1 x 106 cells/vial, 1 mL -
Viability
~90% -
Adherent /Suspension
Adherent -
Tissue
Kidney -
Cell type
epithelial -
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12 -
Antibiotic resistance
Puromycin 1.00µg/ml -
Mycoplasma free
Yes -
Storage instructions
Shipped on Dry Ice. Store in liquid nitrogen. -
Storage buffer
Constituents: 8.7% Dimethylsulfoxide, 2% Cellulose, methyl ether -
Research areas
Images
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Western blot - Human CELF1 knockout HEK293T cell line (ab266086)All lanes : Anti-CUG-BP1 antibody [EPR8298(B)] (ab129115) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : CELF1 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Human brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaLanes 1-4: Merged signal (red and green). Green - ab129115 observed at 52 kDa. Red - loading control ab8245 observed at 36 kDa.
ab129115 Anti-CUG-BP1 antibody [EPR8298(B)] was shown to specifically react with CUG-BP1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266086 (knockout cell lysate ab257390) was used. Wild-type and CUG-BP1 knockout samples were subjected to SDS-PAGE. ab129115 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Western blot - Human CELF1 knockout HEK293T cell line (ab266086)All lanes : Anti-CUG-BP1 antibody [3B1] (ab9549) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : CELF1 knockout HEK293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Human brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDaLanes 1-4: Merged signal (red and green). Green - ab9549 observed at 52 kDa. Red - loading control ab181602 observed at 36 kDa.
ab9549 Anti-CUG-BP1 antibody [3B1] was shown to specifically react with CUG-BP1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266086 (knockout cell lysate ab257390) was used. Wild-type and CUG-BP1 knockout samples were subjected to SDS-PAGE. ab9549 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) and Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human CELF1 knockout HEK293T cell line (ab266086)Allele-1: 5 bp deletion in exon 2
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Sanger Sequencing - Human CELF1 knockout HEK293T cell line (ab266086)Allele-2: 4 bp deletion in exon 2.