Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PSMC5 antibody [EPR13565(B)] (ab178681)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13565(B)] to PSMC5
  • Suitable for: WB, Flow Cyt, ICC/IF, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PSMC5 antibody [EPR13565(B)]
    See all PSMC5 primary antibodies

  • Description

    Rabbit monoclonal [EPR13565(B)] to PSMC5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Rat
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PSMC5 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P62195

  • Positive control

    • WB: HepG2, A431, 293T Mouse heart, Mouse brain, and Rat brain lysate; IHC-P: human lung carcinoma, thyroid carcinoma, mouse liver, and rat liver tissues; ICC/IF: HeLa and PSM5C cells; Flow Cyt: HeLa cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13565(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)
    Western blot - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)
    All lanes : Anti-PSMC5 antibody [EPR13565(B)] (ab178681) at 1/1000 dilution (Purified)

    Lane 1 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
    Lane 2 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates
    Lane 3 : Mouse heart lysates
    Lane 4 : Mouse brain lysates
    Lane 5 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 46 kDa
    Observed band size: 46 kDa

  • Immunoprecipitation - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)
    Immunoprecipitation - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)

    ab178681 (Purified) at 1/40 dilution (2µg) immunoprecipitating PSMC5 in HepG2 whole cell lysate.
    Lane 1 (input): HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate (10µg)
    Lane 2 (+): ab178681 & HepG2 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab178681 in HepG2 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling PSMC5 with Purified ab178681 at 1/2000 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Flow Cytometry - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)
    Flow Cytometry - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PSMC5 with purified ab178681 at 1/90 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)
    Immunocytochemistry/ Immunofluorescence - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PSMC5 with Purifiedab178681 at 1/500 dilution (1.7 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling PSMC5 with Purified ab178681 at 1/2000 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling PSMC5 with Purified ab178681 at 1/2000 dilution (0.43 µg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)
    Western blot - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)
    All lanes : Anti-PSMC5 antibody [EPR13565(B)] (ab178681) at 1/10000 dilution (unpurified)

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 46 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)

    Immunohistochemical analysis of paraffin-embedded Human thyroid carcinoma tissue labeling PSMC5 with unpurified ab178681 at 1/50 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)
    Immunocytochemistry/ Immunofluorescence - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)

    Immunofluorescent analysis of HeLa cells labeling PSMC5 with unpurified ab178681 at 1/100 dilution.

  • Flow Cytometry - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)
    Flow Cytometry - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)

    Flow cytometric analysis of permeabilized Hela cells labeling PSMC5 with unpurified ab178681 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

  • Immunoprecipitation - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)
    Immunoprecipitation - Anti-PSMC5 antibody [EPR13565(B)] (ab178681)

    Western blot analysis on immunoprecipitation pellet from (Lane 1) 293T cell lysate or (Lane 2) 1XPBS (negative control) using unpurified ab178681 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Anti-PSMC5 antibody [EPR13565(B)] (ab178681)
    Anti-PSMC5 antibody [EPR13565(B)] (ab178681)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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