Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸ 10 µl 117 264 ₸

Order now and get it on Tuesday March 02, 2021

Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR13566(B)] to PSMC5 - C-terminal
Suitable for: WB, ICC/IF, Flow Cyt, IHC-P
Reacts with: Human

Product name

Anti-PSMC5 antibody [EPR13566(B)] - C-terminal
See all PSMC5 primary antibodies
Description

Rabbit monoclonal [EPR13566(B)] to PSMC5 - C-terminal
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human PSMC5 aa 350 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P62195
Positive control
- HepG2, HeLa and 293T lysates; Human pancreas tissue; 293T and HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 59% PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR13566(B)
Isotype

IgG
Research areas

Western blot - Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495)

All lanes : Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495) at 1/10000 dilution

Lane 1 : HepG2 lysate
Lane 2 : HeLa lysate
Lane 3 : 293T lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 46 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PSMC5 with ab180495 at 1/250 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG secondary and Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Flow Cytometry - Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495)

Flow Cytometric analysis of HeLa cells (2% paraformaldehyde-fixed) labeling PSMC5 with ab180495 at 1/30 dilution (green) compared to a negative control Rabbit monoclonal IgG (red) follwed by Goat anti rabbit IgG (FITC) secondary at 1/75 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495)

Immunofluorescent analysis of 293T cells (4% paraformaldehyde-fixed) labeling PSMC5 with ab180495 at 1/250 dilution (red) followed by with Goat anti rabbit IgG (Dylight 555) . DAPI staining (blue).
Anti-PSMC5 antibody [EPR13566(B)] - C-terminal (ab180495)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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