Anti-PSMD1 antibody (ab93208)
Key features and details
- Rabbit polyclonal to PSMD1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PSMD1 antibody
See all PSMD1 primary antibodies -
Description
Rabbit polyclonal to PSMD1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human PSMD1 aa 200-300 conjugated to keyhole limpet haemocyanin.
(Peptide available asab105494) -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: K562; HeLa; A431; HepG2; HEK293.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatChickenOrangutanApplication Abreviews Notes WB Use a concentration of 2 µg/ml. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).Notes WB
Use a concentration of 2 µg/ml. Detects a band of approximately 106 kDa (predicted molecular weight: 106 kDa).Target
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Function
Acts as a regulatory subunit of the 26 proteasome which is involved in the ATP-dependent degradation of ubiquitinated proteins. -
Sequence similarities
Belongs to the proteasome subunit S1 family.
Contains 10 PC repeats. - Information by UniProt
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Database links
- Entrez Gene: 424926 Chicken
- Entrez Gene: 5707 Human
- Entrez Gene: 70247 Mouse
- Entrez Gene: 83806 Rat
- SwissProt: Q5F418 Chicken
- SwissProt: Q99460 Human
- SwissProt: Q3TXS7 Mouse
- SwissProt: Q5R5S4 Orangutan
see all -
Alternative names
- MGC133041 antibody
- 26S proteasome non ATPase regulatory subunit 1 antibody
- 26S proteasome non ATPase subunit 1 antibody
see all
Images
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Western blot - Anti-PSMD1 antibody (ab93208)All lanes : Anti-PSMD1 antibody (ab93208) at 2 µg/ml
Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 106 kDa
Observed band size: 106 kDa
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
Datasheets and documents
References (0)
ab93208 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-PSMD1 antibody (ab93208)All lanes : Anti-PSMD1 antibody (ab93208) at 2 µg/ml
Lane 1 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 106 kDa
Observed band size: 106 kDa
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes
