Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8826(2)(B)] to Proteasome subunit beta type 2/PSMB2
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)]
    See all Proteasome subunit beta type 2/PSMB2 primary antibodies

  • Description

    Rabbit monoclonal [EPR8826(2)(B)] to Proteasome subunit beta type 2/PSMB2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Proteasome subunit beta type 2/PSMB2. The exact sequence is proprietary.
    Database link: P49721

  • Positive control

    • Jurkat, HepG2, HeLa and LoVo cell lysates; Human gastric carcinoma and Human liver tissues; Permeabilized Jurkat cells; Immunoprecipitation pellet from HeLa cell lysate.

  • General notes

     This product was previously labelled as PSMB2, Proteasome subunit beta type 2

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR8826(2)(B)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)
    Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)
    All lanes : Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : LoVo cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 23 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)

    Immunohistochemical analysis of paraffin embedded Human liver tissue labeling Proteasome subunit beta type 2/PSMB2 with ab166628 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)

    Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labeling Proteasome subunit beta type 2/PSMB2 with ab166628 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)
    Flow Cytometry - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)
    Flow cytometric analysis of permeabilized Jurkat cells using ab166628 at a 1/100 dilution (red) or a Rabbit IgG (negative) (green).
  • Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)
    Western blot - Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)
    Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628) at 1/1000 dilution + immunoprecipitation pellet from HeLa cell lysate at 10 µg

    Secondary
    HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.

    Predicted band size: 23 kDa

  • Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)
    Anti-Proteasome subunit beta type 2/PSMB2 antibody [EPR8826(2)(B)] (ab166628)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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