Homocitrulline/Citrulline Assay Kit (ab242292)
Key features and details
- Detection method: Colorimetric
- Sample type: Cell Lysate, Plasma, Serum, Tissue Lysate, Urine
Overview
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Product name
Homocitrulline/Citrulline Assay Kit
See all Citrulline kits -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Cell Lysate, Tissue Lysate -
Product overview
The Homocitrulline/Citrulline Assay Kit (ab242292) provides a convenient colorimetric method for the detection of total homocitrulline/citrulline from cells, tissue, plasma, serum, or urine samples.
The content of homocitrulline and citrulline in the unknown samples is determined by comparison with a predetermined standard curve. The provided reagents are sufficient for the evaluation of 100 assays including standards and unknown samples.
Properties
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Storage instructions
Please refer to protocols. -
Components 100 tests Assay Reagent A 1 x 25ml Assay Reagent B 1 x 5ml Citrulline Standard 1 x 50µl Homocitrulline Standard 1 x 50µl Proteinase K 1 x 500µl SDS Solution 1 x 500µl -
Research areas
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Relevance
The amino acid Citrulline is required to detoxify the liver from ammonia, which is a waste product of the body from oxidation. Citrulline promotes energy and assists with the immune system. This unusual amino acid is formed in the urea cycle by the addition of carbon dioxide and ammonia to ornithine. It is then combined with aspartic acid to form arginosuccinic acid, which later is metabolized into the amino acid arginine.
Images
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Homocitrulline standard curve.
Homocitrulline standard curve.
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Citrulline standard curveCitrulline standard curve
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Detection of homocitrulline/citrulline in human urine.Detection of homocitrulline/citrulline in human urine.