Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-USP9Y antibody [EPR13966] (ab181842)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13966] to USP9Y
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-USP9Y antibody [EPR13966]
    See all USP9Y primary antibodies

  • Description

    Rabbit monoclonal [EPR13966] to USP9Y

  • Host species

    Rabbit

  • Specificity

    The immunogen of USP9Y has 78% homology with USP9X (Uniprot: Q93008), which is a homologue of USP9Y on the X chromosome. In female-derived HeLa cells, we found a band which mostly likely to be USP9X. Therefore, this antibody may cross-react with USP9X.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat, K562, Molt-4 and HeLa whole cell lysate (ab150035); HeLa cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-USP9Y antibody [EPR13966] (ab181842)
    Western blot - Anti-USP9Y antibody [EPR13966] (ab181842)
    All lanes : Anti-USP9Y antibody [EPR13966] (ab181842) at 1/20000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Molt-4 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 291 kDa
    Observed band size: 291 kDa

  • Flow Cytometry - Anti-USP9Y antibody [EPR13966] (ab181842)
    Flow Cytometry - Anti-USP9Y antibody [EPR13966] (ab181842)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling USP9Y with ab181842 at 1/200 diltuion (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Anti-USP9Y antibody [EPR13966] (ab181842)
    Anti-USP9Y antibody [EPR13966] (ab181842)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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