Anti-ECM29 antibody (ab28666)
Key features and details
- Rabbit polyclonal to ECM29
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ECM29 antibody -
Description
Rabbit polyclonal to ECM29 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ECM29 aa 1821-1839 (C terminal).
Sequence:RPELQEKAALLKKTLENLE
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Positive control
- WB: HeLa cell lysate. ICC/IF: HeLa cells, K-562 and NTERA-2 cl.D1 lysates
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ECM29 antibody (ab28666)All lanes : Anti-ECM29 antibody (ab28666) at 1/250 dilution
Lane 1 : HeLa whole cell lysate
Lane 2 : K-562 whole cell lysate
Lane 3 : NTERA-2 cl.D1 whole cell lysate
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody at 1/2500 dilution
Predicted band size: 205 kDa
Observed band size: 204 kDa why is the actual band size different from the predicted?Additional bands of 70 and 160 kDa observed might correspond to interacting partners of dynamin intermediate chain and kinesin intermediate chain respectively.
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Immunocytochemistry/ Immunofluorescence - Anti-ECM29 antibody (ab28666)Immunofluorescence analysis of HeLa cells labelling ECM29 with ab28666 at 1/250 dilution. Cells were fixed with 4% paraformaldehyde for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at 1:2000 dilution for 45 minutes at room temperature was used as the secondary antibody (green) (a). F-actin (was stained with Rhodamine Phalloidin (red) (c) and Nuclear DNA was labelled with DAPI (blue) (b). (d) represents the merged image showing cytoplasmic localization. (e) shows the no primary antibody control. The images were captured at 60X magnification.
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Western blot - Anti-ECM29 antibody (ab28666)Anti-ECM29 antibody (ab28666) (dilution of 1/200) + HeLa cell extract
Predicted band size: 205 kDa
Observed band size: 205 kDa
Detection of ECM29 in HeLa cells. -
Immunocytochemistry/ Immunofluorescence - Anti-ECM29 antibody (ab28666)Immunofluorescent staining of Ecm29 in HeLa cells using ab28666 (1/200 dilution).