Anti-PSMD1 antibody (ab140682)
Key features and details
- Rabbit polyclonal to PSMD1
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PSMD1 antibody
See all PSMD1 primary antibodies -
Description
Rabbit polyclonal to PSMD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Non human primates, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Xenopus tropicalis
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Immunogen
Synthetic peptide corresponding to a region between residues 903-953 of Human PSMD1 (NP_002798.2).
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Positive control
- 293T, HeLa, NIH3T3 and Jurkat whole cell lysate (ab7899).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PSMD1 antibody (ab140682)All lanes : Anti-PSMD1 antibody (ab140682) at 0.1 µg/ml
Lane 1 : Whole cell lysate prepared from 293T cells at 50 µg
Lane 2 : Whole cell lysate prepared from 293T cells at 15 µg
Lane 3 : Whole cell lysate prepared from HeLa cells at 50 µg
Lane 4 : Whole cell lysate prepared from Jurkat cells at 50 µg
Developed using the ECL technique.
Predicted band size: 105 kDa
Exposure time: 10 seconds
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Western blot - Anti-PSMD1 antibody (ab140682)Anti-PSMD1 antibody (ab140682) at 0.1 µg/ml + whole cell lysate prepared from NIH3T3 cells at 50 µg
Developed using the ECL technique.
Predicted band size: 105 kDa
Exposure time: 30 seconds
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Immunoprecipitation - Anti-PSMD1 antibody (ab140682)ab140682 at 1 µg/ml detecting PSMD1 in 293T whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes a different epitope of PSMD1.
Lane 2: ab140682 at 6 µg/mg of lysate.
Lane 3: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 3 seconds
