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Epigenetics and Nuclear Signaling Chromatin Binding Proteins DNA / RNA binding

Anti-OBFC2A antibody [OTI6B10] (ab236402)

Anti-OBFC2A antibody [OTI6B10] (ab236402)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [OTI6B10] to OBFC2A
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG2b

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Overview

  • Product name

    Anti-OBFC2A antibody [OTI6B10]
    See all OBFC2A primary antibodies
  • Description

    Mouse monoclonal [OTI6B10] to OBFC2A
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human OBFC2A aa 1 to the C-terminus. Produced in E.coli (NP_001026886).
    Database link: Q96AH0

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: HEK-293T cell lysate transfected with pCMV6-ENTRY OBFC2A cDNA for 48 hrs; HeLa cell lysate. IHC-P: Human breast, colon adenocarcinoma, kidney, thyroid, endometrium, prostate and bladder carcinoma tissues.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Affinity purified
  • Purification notes

    Purified from cell culture supernatant by affinity chromatography.
  • Clonality

    Monoclonal
  • Clone number

    OTI6B10
  • Isotype

    IgG2b
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Images

  • Western blot - Anti-OBFC2A antibody [OTI6B10] (ab236402)
    Western blot - Anti-OBFC2A antibody [OTI6B10] (ab236402)
    All lanes : Anti-OBFC2A antibody [OTI6B10] (ab236402) at 1/2000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs
    Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY OBFC2A cDNA for 48 hrs

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 22 kDa

  • Western blot - Anti-OBFC2A antibody [OTI6B10] (ab236402)
    Western blot - Anti-OBFC2A antibody [OTI6B10] (ab236402)
    Anti-OBFC2A antibody [OTI6B10] (ab236402) at 1/500 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 35 µg

    Predicted band size: 22 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBFC2A antibody [OTI6B10] (ab236402)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBFC2A antibody [OTI6B10] (ab236402)

    Paraffin-embedded human breast tissue stained for OBFC2A using ab236402 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBFC2A antibody [OTI6B10] (ab236402)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBFC2A antibody [OTI6B10] (ab236402)

    Paraffin-embedded human colon adenocarcinoma tissue stained for OBFC2A using ab236402 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBFC2A antibody [OTI6B10] (ab236402)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBFC2A antibody [OTI6B10] (ab236402)

    Paraffin-embedded human kidney tissue stained for OBFC2A using ab236402 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBFC2A antibody [OTI6B10] (ab236402)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBFC2A antibody [OTI6B10] (ab236402)

    Paraffin-embedded human thyroid tissue stained for OBFC2A using ab236402 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBFC2A antibody [OTI6B10] (ab236402)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBFC2A antibody [OTI6B10] (ab236402)

    Paraffin-embedded human endometrium tissue stained for OBFC2A using ab236402 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBFC2A antibody [OTI6B10] (ab236402)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBFC2A antibody [OTI6B10] (ab236402)

    Paraffin-embedded human prostate tissue stained for OBFC2A using ab236402 at 1/500 dilution in immunohistochemical analysis.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBFC2A antibody [OTI6B10] (ab236402)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OBFC2A antibody [OTI6B10] (ab236402)

    Paraffin-embedded human bladder carcinoma tissue stained for OBFC2A using ab236402 at 1/500 dilution in immunohistochemical analysis.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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