Anti-OBFC2A antibody [OTI6B10] (ab236402)
Key features and details
- Mouse monoclonal [OTI6B10] to OBFC2A
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-OBFC2A antibody [OTI6B10]
See all OBFC2A primary antibodies -
Description
Mouse monoclonal [OTI6B10] to OBFC2A -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human OBFC2A aa 1 to the C-terminus. Produced in E.coli (NP_001026886).
Database link: Q96AH0 -
Positive control
- WB: HEK-293T cell lysate transfected with pCMV6-ENTRY OBFC2A cDNA for 48 hrs; HeLa cell lysate. IHC-P: Human breast, colon adenocarcinoma, kidney, thyroid, endometrium, prostate and bladder carcinoma tissues.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography. -
Clonality
Monoclonal -
Clone number
OTI6B10 -
Isotype
IgG2b -
Research areas
Images
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All lanes : Anti-OBFC2A antibody [OTI6B10] (ab236402) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA for 48 hrs
Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY OBFC2A cDNA for 48 hrs
Lysates/proteins at 5 µg per lane.
Predicted band size: 22 kDa
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Anti-OBFC2A antibody [OTI6B10] (ab236402) at 1/500 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 35 µg
Predicted band size: 22 kDa
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Paraffin-embedded human breast tissue stained for OBFC2A using ab236402 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human colon adenocarcinoma tissue stained for OBFC2A using ab236402 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human kidney tissue stained for OBFC2A using ab236402 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human thyroid tissue stained for OBFC2A using ab236402 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human endometrium tissue stained for OBFC2A using ab236402 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human prostate tissue stained for OBFC2A using ab236402 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded human bladder carcinoma tissue stained for OBFC2A using ab236402 at 1/500 dilution in immunohistochemical analysis.