Aggresome Detection Kit (ab139486)
Key features and details
- Assay type: Cell-based
- Detection method: Fluorescent
- Platform: Flow cytometer, Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Aggresome Detection Kit -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based -
Product overview
Abcam's Aggresome Detection kit (ab139486) provides a rapid, specific and quantitative approach to identify inhibitors relevant to neurodegenerative disease in an authentic cellular context. This fixed-cell assay doesn’t require non-physiological protein mutations or genetically engineered cell lines. The Aggresome Detection Reagent has been validated under a wide range of conditions and with small molecule modulators, demonstrating suitability for screening compounds of potential therapeutic value, and optimized for antibody co-localization studies to identify interactions between aggregated protein cargo and the various proteins implicated in autophagy and aggresome formation. Suggested uses for this kit include:
1. The identification of small molecules that inhibit aggresome formation.
2. Immuno-localization studies between aggregated protein cargo and the various proteins implicated in aggresome formation, such as histone deacetylase 6, parkin, ataxin-3, dynein motor complex and ubiquilin-1.
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Platform
Flow cytometer, Fluorescence microscope
Properties
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Storage instructions
Please refer to protocols. -
Components 100 tests 10X Assay Buffer 1 x 25ml Aggresome Detection Reagent 1 x 10µl Hoechst 33342 Nuclear Stain 1 x 50µl Proteasome Inhibitor 1 vial -
Research areas
Images
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Aggresome Detection Kit (ab139486)
The increase in the fluorescence intensity of the Aggresome Detection Reagent with the addition of MG-132 in Jurkat cells. Jurkat cells were mock induced with 0.08% DMSO (blue line) or induced with 8 μM MG-132 (black line) in a 37ºC, 5% CO2 incubator for 24 hours.
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Aggresomes detected by Aggresome Detection Reagent in HeLa cells.Aggresomes were detected in HeLa cells after overnight incubation with a range of proteasome inhibitors, as observed by fluorescence microscopy.
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Excitation and fluorescence emission spectra for Aggresome Detection Reagent and Hoechst 33342Excitation and fluorescence emission spectra for Aggresome reagent dye, Ex/Em=500/600 nm (panel A, left) and Hoechst 33342, Ex/Em=350/461 nm (panel B, right). All spectra were determined in 1X Assay Buffer.