Anti-ESD antibody [EPR8446] (ab131211)
![Anti-ESD antibody [EPR8446] (ab131211)](https://www.abcam.com/ps/products/131/ab131211/Images/ab131211-412765-anti-esd-antibody-epr8446-western-blot-human-knockout-lysate.jpg "Anti-ESD antibody [EPR8446] (ab131211)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8446] to ESD
- Suitable for: WB, Flow Cyt
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-ESD antibody [EPR8446]
See all ESD primary antibodies -
Description
Rabbit monoclonal [EPR8446] to ESD -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide from a region of Human ESD (P10768)
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Positive control
- WB: HEK293T, K562, HeLa, Jurkat and Caco-2 cell lysates. Flow Cyt: Jurkat cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8446 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ESD antibody [EPR8446] (ab131211)All lanes : Anti-ESD antibody [EPR8446] (ab131211) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : ESD knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 31 kDa
Observed band size: 31 kDaLanes 1-4: Merged signal (red and green). Green - ab131211 observed at 31 kDa. Red - loading control ab8245 observed at 36 kDa.
ab131211 Anti-ESD antibody [EPR8446] was shown to specifically react with ESD in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266360 (knockout cell lysate ab257942) was used. Wild-type and ESD knockout samples were subjected to SDS-PAGE. ab131211 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Flow Cytometry - Anti-ESD antibody [EPR8446] (ab131211)](https://www.abcam.com/ps/products/131/ab131211/Images/ESD-Primary-antibodies-ab131211-2.jpg)
Flow Cytometry - Anti-ESD antibody [EPR8446] (ab131211)ab131211 at 1/10 labelling ESD in permeabilized Jurkat cells by flow cytometry. Rabbit IgG negative control is in green. -
![Western blot - Anti-ESD antibody [EPR8446] (ab131211)](https://www.abcam.com/ps/products/131/ab131211/Images/ESD-Primary-antibodies-ab131211-1.jpg)
Western blot - Anti-ESD antibody [EPR8446] (ab131211)All lanes : Anti-ESD antibody [EPR8446] (ab131211) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 31 kDa
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![Anti-ESD antibody [EPR8446] (ab131211)](https://www.abcam.com/ps/products/131/ab131211/Images/ab131211-4-benefits-of-recombinant-antibodies.png)
Anti-ESD antibody [EPR8446] (ab131211)
