Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70)
![Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70)](https://www.abcam.com/ps/products/0/ab70/Images/ab70-334509-anti-hpv16-e6-hpv18-e6-antibody-c1p5-immunohistochemistry.jpg "Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70)")
Key features and details
- Mouse monoclonal [C1P5] to HPV16 E6 + HPV18 E6
- Suitable for: WB, IP, IHC-P
- Reacts with: Human papillomavirus
- Isotype: IgG1
Overview
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Product name
Anti-HPV16 E6 + HPV18 E6 antibody [C1P5]
See all HPV16 E6 + HPV18 E6 primary antibodies -
Description
Mouse monoclonal [C1P5] to HPV16 E6 + HPV18 E6 -
Host species
Mouse -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human papillomavirus -
Immunogen
Gel-purified HPV-18 E6-beta galactosidase fusion protein.
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Positive control
- Human cervical cancer cells.
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General notes
Clinical relevance: Detection of HPV in cervical smears and biopsies. Uses: Analysis of E6 expression in cell transformation studies.
The E6 proteins are known to form oligomers; PMID19917295, PMID17209544.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
C1P5 -
Myeloma
NS1 -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70) Image courtesy of Mr Allan Wang
Formalin-fixed, paraffin-embedded human uterine cervix squamous cell carcinoma tissue stained for HPV16 E6 + HPV18 E6 using ab70 at 1/200 dilution in immunohistochemical analysis.
Incubated with primary antibody for 1 hour at 25°C.
Heat mediated antigen retrieval at pH 6.
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![Western blot - Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70)](https://www.abcam.com/ps/products/0/ab70/Images/ab70-3282-anti-hpv16-e6-hpv18-e6-antibody-c1p5-western-blot.jpg)
Western blot - Anti-HPV16 E6 + HPV18 E6 antibody [C1P5] (ab70) Image from Zhang Y et al, J Biol Chem. 2005 Sep 30;280(39):33165-77. Epub 2005 Jun 27, Fig 9. DOI 10.1074/jbc.M505124200Subconfluent proliferating MCF-7 or T47D cells were transiently transfected overnight with empty pcDNA3 vector, with FLAG wild-type E6 or zinc finger defective mutant E6 (E6-(C66,136G)). The cells were washed, post-incubated for 24 hours to allow gene expression, and harvested for Western blotting to detect the E6 oncoprotein using ab70.
