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Microbiology Organism Virus DNA Virus double stranded DNA Virus Human Papillomavirus

Anti-HPV11 E7 antibody [9H5] (ab100967)

Price and availability

274 732 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-HPV11 E7 antibody [9H5] (ab100967)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [9H5] to HPV11 E7
  • Suitable for: WB, IP, ELISA, ICC/IF
  • Reacts with: Human papillomavirus
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-HPV11 E7 antibody [9H5]
  • Description

    Mouse monoclonal [9H5] to HPV11 E7
  • Host species

    Mouse
  • Specificity

    Reacts in WB, IP, ELISA with HPV11 native E7 protein as well as recombinant E7 protein expressed in bacteria and Cos7 cells.
  • Tested applications

    Suitable for: WB, IP, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Human papillomavirus
  • Immunogen

    Recombinant full length HPV11 E7 protein purified from bacteria.

  • Epitope

    Mapped to amino acids 36-70
  • Positive control

    • WB: U2OS and HeLa cell lysates ICC/IF: U2OS cells transfected with plasmid expressing HPV-11 E7 protein IP: lysate of U20S cells
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    9H5
  • Isotype

    IgG2a
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • Human Papillomavirus

Images

  • Western blot - Anti-HPV11 E7 antibody [9H5] (ab100967)
    Western blot - Anti-HPV11 E7 antibody [9H5] (ab100967)
    All lanes : Anti-HPV11 E7 antibody [9H5] (ab100967) at 1/1000 dilution

    Lanes 1 & 4 : lysate of U20S cells transfected with plasmid expressing HPV11 E7 protein
    Lane 2 : lysate of plain U20S cells
    Lane 3 : lysate of HeLa cells
    Lane 5 : marker

    Predicted band size: 11 kDa

  • Immunoprecipitation - Anti-HPV11 E7 antibody [9H5] (ab100967)
    Immunoprecipitation - Anti-HPV11 E7 antibody [9H5] (ab100967)
    Immunoprecipitation (IP) analysis of HPV11 E7 protein.
    Lane 1 represents the marker.
    Lane 2 shows detection of E7 protein by ab100967 in lysate of U20S cells.
    In lane 3 the lower band is precipitated E7, upper bands are light and heavy chain of the antibody.
    Lane 4 is precipitation without ab100967
    Lane 5 shows IP experiment with plain U2OS cells.
  • Immunocytochemistry/ Immunofluorescence - Anti-HPV11 E7 antibody [9H5] (ab100967)
    Immunocytochemistry/ Immunofluorescence - Anti-HPV11 E7 antibody [9H5] (ab100967)
    Immunofluorescence (IF) analysis of HPV11 E7 protein in U2OS cells.
    IF was carried out using U2OS cells transfected with plasmid expressing HPV11 E7 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV11 E7 detected by ab100967. IF analysis of E7 protein shows similar result also in HeLa cells.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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