Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free (ab248114)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4140(2)] to SCRIBBLE - BSA and Azide free
  • Suitable for: ICC, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free
    See all SCRIBBLE primary antibodies

  • Description

    Rabbit monoclonal [EPR4140(2)] to SCRIBBLE - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, Flow Cyt, WBmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293T, HeLa and MCF7 cell lysates; permeabilized 293T cells.

  • General notes

    Ab248114 is the carrier-free version of ab125080. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248114 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free (ab248114)
    Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free (ab248114)
    All lanes : Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080) at 1/500 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : SCRIB knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 175 kDa
    Observed band size: 240 kDa
    why is the actual band size different from the predicted?



    This data was developed using 125080, the same antibody clone in a different buffer formulation.

    Lanes 1-2: Merged signal (red and green). Green - ab125080 observed at 240 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab125080 Anti-SCRIBBLE antibody [EPR4140(2)]  was shown to specifically react with SCRIBBLE in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265190 (knockout cell lysate ab257660) was used. Wild-type and SCRIBBLE knockout samples were subjected to SDS-PAGE. ab125080 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free (ab248114)
    Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free (ab248114)
    All lanes : Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 175 kDa



    This data was developed using ab125080, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free (ab248114)
    Flow Cytometry - Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free (ab248114)
    This data was developed using ab125080, the same antibody clone in a different buffer formulation.ab125080 at 1/100, staining SCRIBBLE in permeabilized 293T cells by flow cytometry (red), compared with a negative control Rabbit IgG antibody (green).
  • Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free (ab248114)
    Anti-SCRIBBLE antibody [EPR4140(2)] - BSA and Azide free (ab248114)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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