Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4140(2)] to SCRIBBLE
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-SCRIBBLE antibody [EPR4140(2)]
    See all SCRIBBLE primary antibodies

  • Description

    Rabbit monoclonal [EPR4140(2)] to SCRIBBLE

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human SCRIBBLE aa 1550-1650 (C terminal). The exact sequence is proprietary.

  • Positive control

    • 293T, HeLa and MCF7 cell lysates; permeabilized 293T cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR4140(2)

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
    Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
    All lanes : Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080) at 1/500 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : SCRIB knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 175 kDa
    Observed band size: 240 kDa
    why is the actual band size different from the predicted?



    Lanes 1-2: Merged signal (red and green). Green - ab125080 observed at 240 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab125080 Anti-SCRIBBLE antibody [EPR4140(2)]  was shown to specifically react with SCRIBBLE in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265190 (knockout cell lysate ab257660) was used. Wild-type and SCRIBBLE knockout samples were subjected to SDS-PAGE. ab125080 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
    Western blot - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
    All lanes : Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 175 kDa

  • Flow Cytometry - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
    Flow Cytometry - Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
    ab125080 at 1/100, staining SCRIBBLE in permeabilized 293T cells by flow cytometry (red), compared with a negative control Rabbit IgG antibody (green).
  • Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)
    Anti-SCRIBBLE antibody [EPR4140(2)] (ab125080)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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