Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ESD antibody [EPR8446] - BSA and Azide free (ab248372)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8446] to ESD - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ESD antibody [EPR8446] - BSA and Azide free
    See all ESD primary antibodies

  • Description

    Rabbit monoclonal [EPR8446] to ESD - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, K562, HeLa, Jurkat and Caco-2 cell lysates. Flow Cyt: Jurkat cells.

  • General notes

    ab248372 is the carrier-free version of ab131211 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab248372 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ESD antibody [EPR8446] - BSA and Azide free (ab248372)
    Western blot - Anti-ESD antibody [EPR8446] - BSA and Azide free (ab248372)
    All lanes : Anti-ESD antibody [EPR8446] (ab131211) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : ESD knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate
    Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 31 kDa
    Observed band size: 31 kDa



    This data was developed using ab131211, the same antibody clone in a different buffer formulation.

    Lanes 1-4: Merged signal (red and green). Green - ab131211 observed at 31 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab131211 Anti-ESD antibody [EPR8446] was shown to specifically react with ESD in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266360 (knockout cell lysate ab257942) was used. Wild-type and ESD knockout samples were subjected to SDS-PAGE. ab131211 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-ESD antibody [EPR8446] - BSA and Azide free (ab248372)
    Flow Cytometry - Anti-ESD antibody [EPR8446] - BSA and Azide free (ab248372)
    This data was developed using ab131211, the same antibody clone in a different buffer formulation.ab131211 at 1/10 labelling ESD in permeabilized Jurkat cells by flow cytometry. Rabbit IgG negative control is in green.
  • Western blot - Anti-ESD antibody [EPR8446] - BSA and Azide free (ab248372)
    Western blot - Anti-ESD antibody [EPR8446] - BSA and Azide free (ab248372)
    All lanes : Anti-ESD antibody [EPR8446] (ab131211) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Caco-2 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 31 kDa



    This data was developed using ab131211, the same antibody clone in a different buffer formulation.

  • Anti-ESD antibody [EPR8446] - BSA and Azide free (ab248372)
    Anti-ESD antibody [EPR8446] - BSA and Azide free (ab248372)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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