Ethanol Assay Kit (ab272531)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Sample type: Cell culture media, Food samples, Plasma, Saliva, Serum, Urine
Overview
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Product name
Ethanol Assay Kit
See all Ethanol kits -
Detection method
Colorimetric -
Sample type
Saliva, Urine, Serum, Plasma, Cell culture media, Food samples -
Assay type
Quantitative -
Range
0.04 % - 4 % -
Product overview
Ethanol Assay Kit (ab272531) is a simple, direct and automation-ready procedure for measuring ethanol concentration. This assay kit is based on an improved dichromate method, in which dichromate is reduced by ethanol to a bluish chromic (Cr3+) product. The intensity of color, measured at 580 nm, is a direct measure of the alcohol concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples and exhibits high sensitivity.
Sensitive and accurate: Detection range 0.04 – 2% alcohol in 96-well plate assay.
Convenient and high-throughput: The procedure involves adding a single working reagent, incubation for 8 min, adding a Stop Reagent, and reading the optical density. Can be readily automated as a highthroughput 96-well plate assay for thousands of samples per day.
Versatility: Assays can be executed in 96-well plate or cuvet.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: Functional Studiesmore details
Properties
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Storage instructions
Please refer to protocols. -
Components 500 tests Reagent A 1 x 50ml Reagent B 1 x 50ml Standard 1 x 1.5ml TCA (10%) 1 x 50ml -
Research areas
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Relevance
Alcohol (ethanol C2H5OH) is a clear colorless liquid rapidly absorbed from the gastrointestinal tract and distributed through the body. Ethanol has a depressive effect on the central nervous system and because of its psychoactive effects, it is considered a drug. Ethanol is metabolized by the body as an enerfy-providing carbohydrate nutrient, as it metabolized into acetyl CoA, an intermediate common with glucose than can be used for energy in the citric acid cycle (TCA) or for biosynthesis. Ethanol within the human body is converted into acetaldehyde by alcohol dehydrogenase and then into acetic acid by acetaldehyde dehydriogenase. Acetaldehyde, the product of the first step of the alcohol breakdown, is linked to most of the toxic clinical effects of alcohol. -
Alternative names
- Ethyl alcohol
Images
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Ethanol Assay Kit standard curve in 96-well plate
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.