Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Orai3 antibody [EPR22575-17] (ab254260)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22575-17] to Orai3
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Orai3 antibody [EPR22575-17]
    See all Orai3 primary antibodies

  • Description

    Rabbit monoclonal [EPR22575-17] to Orai3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human kidney and testis tissue lysates; RAW 264.7, NIH/3T3, MCF7 and A549 whole cell lysates. IHC-P: Human breast cancer, lung cancer and colon cancer tissue; mouse colon tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22575-17

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Orai3 antibody [EPR22575-17] (ab254260)
    Western blot - Anti-Orai3 antibody [EPR22575-17] (ab254260)
    All lanes : Anti-Orai3 antibody [EPR22575-17] (ab254260) at 1/1000 dilution

    Lane 1 : Human kidney lysate at 20 µg
    Lane 2 : Human testis lysate at 20 µg
    Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
    Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate at 20 µg
    Lane 5 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
    Lane 6 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg

    Secondary
    Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
    Lanes 3-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 31 kDa
    Observed band size: 33 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID:26654173, 30123430).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] (ab254260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] (ab254260)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Orai3 with ab254260 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in mouse colon (PMID:30123430 ) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] (ab254260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] (ab254260)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue (A) and its adjacent non-cancerous tissue (B) labeling Orai3 with ab254260 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong membranous staining in human colon cancer tissue (A) while weak staining in its adjacent noncancerous tissue (B) (PMID:30123430 ) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] (ab254260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] (ab254260)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue (A) and its adjacent non-cancerous tissue (B) labeling Orai3 with ab254260 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong membranous staining in human lung cancer tissue (A) while weak staining in its adjacent noncancerous tissue (B) (PMID:24058448 ) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] (ab254260)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] (ab254260)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue (A) and its adjacent non-cancerous tissue (B) labeling Orai3 with ab254260 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong membranous staining in human breast cancer tissue (A) while weak staining in the adjacent noncancerous tissue (B) (PMID: 23266555) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-Orai3 antibody [EPR22575-17] (ab254260)
    Anti-Orai3 antibody [EPR22575-17] (ab254260)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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