Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Thursday March 04, 2021

Anti-Orai3 antibody [EPR22575-17] (ab254260)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22575-17] to Orai3
Suitable for: WB, IHC-P
Reacts with: Mouse, Human

Product name

Anti-Orai3 antibody [EPR22575-17]
See all Orai3 primary antibodies
Description

Rabbit monoclonal [EPR22575-17] to Orai3
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Mouse Human
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human kidney and testis tissue lysates; RAW 264.7, NIH/3T3, MCF7 and A549 whole cell lysates. IHC-P: Human breast cancer, lung cancer and colon cancer tissue; mouse colon tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22575-17
Isotype

IgG
Research areas

Western blot - Anti-Orai3 antibody [EPR22575-17] (ab254260)

All lanes : Anti-Orai3 antibody [EPR22575-17] (ab254260) at 1/1000 dilution

Lane 1 : Human kidney lysate at 20 µg
Lane 2 : Human testis lysate at 20 µg
Lane 3 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 4 : A549 (human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 5 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
Lane 6 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg

Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 3-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 31 kDa
Observed band size: 33 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID:26654173, 30123430).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] (ab254260)

Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Orai3 with ab254260 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in mouse colon (PMID:30123430 ) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] (ab254260)

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue (A) and its adjacent non-cancerous tissue (B) labeling Orai3 with ab254260 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong membranous staining in human colon cancer tissue (A) while weak staining in its adjacent noncancerous tissue (B) (PMID:30123430 ) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] (ab254260)

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue (A) and its adjacent non-cancerous tissue (B) labeling Orai3 with ab254260 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong membranous staining in human lung cancer tissue (A) while weak staining in its adjacent noncancerous tissue (B) (PMID:24058448 ) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] (ab254260)

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue (A) and its adjacent non-cancerous tissue (B) labeling Orai3 with ab254260 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong membranous staining in human breast cancer tissue (A) while weak staining in the adjacent noncancerous tissue (B) (PMID: 23266555) is observed. Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-Orai3 antibody [EPR22575-17] (ab254260)

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