Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP880Y] to EEF2/Elongation factor 2 - BSA and Azide free
  • Suitable for: IHC-P, WB, IP, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free
    See all EEF2/Elongation factor 2 primary antibodies

  • Description

    Rabbit monoclonal [EP880Y] to EEF2/Elongation factor 2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247388 is the carrier-free version of ab75748 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247388 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as EEF2

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP880Y

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)
    Western blot - Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)
    All lanes : Anti-EEF2/Elongation factor 2 antibody [EP880Y] (ab75748) at 1/50000 dilution

    Lane 1 : C6 (rat glioma) whole cell lysates
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 3 : NIH/3T3 (mouse embryo) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Rabbit monoclonal [EP880Y] to EEF2/Elongation factor 2 (ab75748) at 1/100000 dilution

    Predicted band size: 95 kDa



    This data was developed using ab75748, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)

    This data was developed using ab75748, the same antibody clone in a different buffer formulation.

    ab75748 staining EEF2/Elongation factor 2 in rat pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/700. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500. Negative control 1: PBS in place of primary antibody.
  • Immunocytochemistry - Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)
    Immunocytochemistry - Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)

    This data was developed using ab75748, the same antibody clone in a different buffer formulation.

    ab75748 staining EEF2/Elongation factor 2 in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 and ab150120 were used as counterstains for primary antibody ab75748 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain. Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)
  • Flow Cytometry - Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)
    Flow Cytometry - Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)

    This data was developed using ab75748, the same antibody clone in a different buffer formulation.

    ab75748 staining EEF2/Elongation factor 2 in the human cell line HEK293 (human embryonic kidney) by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/70. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/500 was used as the secondary antibody. Isoytype control: Rabbit monoclonal IgG (Black) Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
  • Immunoprecipitation - Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)
    Immunoprecipitation - Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)

    ab75748&

    44; the same antibody clone in a different buffer formulation.

    >ab75748 immunoprecipitating EEF2/Elongation factor 2. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000.

    Lane 1: HeLa (human cervix adenocarcinoma) whole cell lysate (10µg).

    Lane 2: HeLa (human cervix adenocarcinoma) whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab75748 in HeLa (human cervix adenocarcinoma) whole cell lysate.

  • Western blot - Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)
    Western blot - Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)
    All lanes : Anti-EEF2/Elongation factor 2 antibody [EP880Y] (ab75748) at 1/50000 dilution

    Lane 1 : HEK293 (human embryonic kidney) whole cell lysates
    Lane 2 : HeLa (human cervix adenocarcinoma) whole cell lysates
    Lane 3 : A431 (human epidermoid carcinoma) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 95 kDa
    Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.



    This data was developed using ab75748, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)

    This data was developed using ab75748, the same antibody clone in a different buffer formulation.

    ab75748 staining EEF2/Elongation factor 2 in mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody. Negative control 1: PBS in place of primary antibody.
  • Flow Cytometry - Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)
    Flow Cytometry - Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)

    This data was developed using ab75748, the same antibody clone in a different buffer formulation.

    Overlay histogram showing HeLa cells stained with ab75748 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75748, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Immunoprecipitation - Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)
    Immunoprecipitation - Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)
    This data was developed using ab75748, the same antibody clone in a different buffer formulation.EEF2/Elongation factor 2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit monoclonal to EEF2/Elongation factor 2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab75748.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 95kDa; EEF2/Elongation factor 2
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)
    This data was developed using ab75748, the same antibody clone in a different buffer formulation.ab75748 staining EEF2/Elongation factor 2 in human glioma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody. Negative control 1: PBS in place of primary antibody.
  • Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)
    Anti-EEF2/Elongation factor 2 antibody [EP880Y] - BSA and Azide free (ab247388)

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