Anti-Orai3 antibody (ab224095)
Key features and details
- Rabbit polyclonal to Orai3
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Orai3 antibody
See all Orai3 primary antibodies -
Description
Rabbit polyclonal to Orai3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Orai3 aa 150-250.
Database link: Q9BRQ5 -
Positive control
- WB: HEK-293T overexpressing Orai3 cell lysate. IHC-P: Human testis tissue. ICC/IF: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Orai3 antibody (ab224095)All lanes : Anti-Orai3 antibody (ab224095) at 1/100 dilution
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) overexpressing Orai3 (co-expressed with a C-terminal myc-DDK tag), cell lysate
Predicted band size: 31 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody (ab224095)Paraffin-embedded human testis tissue stained for Orai3 using ab224095 at 1/50 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Orai3 antibody (ab224095)PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for Orai3 (green) using ab224095 at 4 µg/ml in ICC/IF.
