Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free (ab238426)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9392] to SERCA2 ATPase - BSA and Azide free
  • Suitable for: WB, Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free
    See all SERCA2 ATPase primary antibodies

  • Description

    Rabbit monoclonal [EPR9392] to SERCA2 ATPase - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa and HepG2 whole cell lysates. Rat and mouse brain tissue lysates; ICC/IF: HeLa and HepG2 cells; IHC-P: Human kidney, liver, brain, and lung tissue. Flow-cyt: HepG2 cells.

  • General notes

    Ab238426 is the carrier-free version of ab150435. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab238426 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free (ab238426)
    Western blot - Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free (ab238426)
    All lanes : Anti-SERCA2 ATPase antibody [EPR9392] (ab150435) at 1/5000 dilution (Purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates boiled
    Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates unboiled
    Lane 3 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates boiled
    Lane 4 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates unboiled
    Lane 5 : Mouse brain lysates boiled
    Lane 6 : Mouse brain lysates unboiled
    Lane 7 : Rat brain lysates boiled
    Lane 8 : Rat brain lysates unboiled

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 115 kDa
    Observed band size: 115,140 kDa
    why is the actual band size different from the predicted?



    Suggest to use non-boiled samples, as boiling process could cause membrane protein aggregates (PMID: 16023741 and PMID: 8670158).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150435).

  • Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free (ab238426)
    Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free (ab238426)

    Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling SERCA2 ATPase with Purified ab150435 at 1/100 dilution (10 µg/mL). Cells were fixed in 100% Methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150435).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free (ab238426)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free (ab238426)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human lung carcinoma tissue sections labeling SERCA2 ATPase with Purified ab150435 at 1/50 dilution (20 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150435).
  • Flow Cytometry - Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free (ab238426)
    Flow Cytometry - Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free (ab238426)
    Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling SERCA2 ATPase with Purified ab150435 at 1/1000 dilution (1 µg/mL) (Red). Cells were fixed with 80% Methanol and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150435).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free (ab238426)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free (ab238426)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human brain tissue sections labeling SERCA2 ATPase with Purified ab150435 at 1/50 dilution (20 µg/mL). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150435).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free (ab238426)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free (ab238426)

    Immunohistochemical analysis of paraffin embedded Human liver tissue labelling SERCA2 ATPase with unpurified ab150435 at 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150435).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free (ab238426)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free (ab238426)

    Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling SERCA2 ATPase with unpurified ab150435 at 1/50.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150435).

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free (ab238426)
    Flow Cytometry - Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free (ab238426)

    Overlay histogram showing HepG2 cells stained with unpurified ab150435 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150435, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150435).

  • Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free (ab238426)
    Immunocytochemistry/ Immunofluorescence - Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free (ab238426)

    Immunofluorescence analysis of HeLa cells labelling SERCA2 ATPase with unpurified ab150435 at 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab150435).

  • Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free (ab238426)
    Anti-SERCA2 ATPase antibody [EPR9392] - BSA and Azide free (ab238426)

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