Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SERCA2 ATPase antibody [IID8] (ab2817)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [IID8] to SERCA2 ATPase
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-SERCA2 ATPase antibody [IID8]
    See all SERCA2 ATPase primary antibodies

  • Description

    Mouse monoclonal [IID8] to SERCA2 ATPase

  • Host species

    Mouse

  • Specificity

    Detects Sarcoplasmic or Endoplasmic Reticulum Calcium 2 (SERCA2) ATPase. This antibody does not discriminate between the two isoforms. By Western blot, this antibody detects an ~110 kDa protein representing SERCA2 ATPase from canine skeletal muscle triad preparations. Immunofluorescence staining of SECRA2 ATPase in rabbit skeletal muscle results in strong labeling of the entire type I (slow) myofiber consistent with sarcoplasmic reticulum localization. This antibody is not recommended for Western blot detection of rat SERCA2.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Full length native protein (purified) corresponding to Dog SERCA2 ATPase. Purified canine cardiac sarcoplasmic reticulum.

  • Positive control

    • IHC-P: Human skeletal muscle and cardiac muscle tissue. WB: Human skeletal muscle. HeLa, HepG2, A549 and A673 whole cell lysate.

  • General notes

    This product has switched from a hybridoma to recombinant production method on 25th November 2020.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [IID8] (ab2817)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [IID8] (ab2817)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling SERCA2 ATPase with ab2817 at 1/4000 dilution, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human skeletal muscle tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [IID8] (ab2817)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SERCA2 ATPase antibody [IID8] (ab2817)

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling SERCA2 ATPase with ab2817 at 1/4000 dilution, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human cardiac muscle tissue is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).
    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-SERCA2 ATPase antibody [IID8] (ab2817)
    Western blot - Anti-SERCA2 ATPase antibody [IID8] (ab2817)
    All lanes : Anti-SERCA2 ATPase antibody [IID8] (ab2817) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 3 : A549 (human lung carcinoma epithelial cell), whole cell lysate
    Lane 4 : A673 (human muscle Ewing's Sarcoma), whole cell lysate
    Lane 5 : Human skeletal muscle

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/100000 dilution

    Observed band size: 110 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lanes 1-2: 92 seconds; Lanes 3-4: 10 seconds; Lane 5: 3 seconds.

    Samples are non-boiled as boiling may cause protein aggregates.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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