Antibodies, Proteins, Kits and Reagents for Life Science

264 681 ₸ Product size

100 µl 264 681 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Gbeta5 antibody [EPR14778] (ab185206)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14778] to Gbeta5
Suitable for: WB, ICC/IF, IHC-P, IP, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-Gbeta5 antibody [EPR14778]
See all Gbeta5 primary antibodies
Description

Rabbit monoclonal [EPR14778] to Gbeta5
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Mouse Human
IP	Rat
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human cerebellum, rat and mouse brain lysates; Human lung adenocarcinoma and mouse brain tissues; HACAT and U87-MG cells.
General notes
Previously labelled as GNB5.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14778
Isotype

IgG
Research areas

Western blot - Anti-Gbeta5 antibody [EPR14778] (ab185206)

Anti-Gbeta5 antibody [EPR14778] (ab185206) at 1/2000 dilution + Human cerebellum lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gbeta5 antibody [EPR14778] (ab185206)

Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling Gbeta5 with ab185206 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Gbeta5 antibody [EPR14778] (ab185206)

Western blot analysis of Gbeta5 in immunoprecipitation pellets from Rat brain lysate using ab185206 at 1/50 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.
Flow Cytometry - Anti-Gbeta5 antibody [EPR14778] (ab185206)

Flow cytometry analysis of 2% paraformaldehyde fixed HACAT cells labeling Gbeta5 with ab185206 at 1/160 dilution (red) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Gbeta5 antibody [EPR14778] (ab185206)

Immunofluorescent analysis of 4% paraformaldehyde-fixed U87-MG cells labeling Gbeta5 with ab185206 at 1/100 dilution. Secondary antibody: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Counterstained with Dapi blue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gbeta5 antibody [EPR14778] (ab185206)

Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling Gbeta5 with ab185206 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-Gbeta5 antibody [EPR14778] (ab185206)

Anti-Gbeta5 antibody [EPR14778] (ab185206) at 1/10000 dilution + Mouse brain lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa
Western blot - Anti-Gbeta5 antibody [EPR14778] (ab185206)

Anti-Gbeta5 antibody [EPR14778] (ab185206) at 1/10000 dilution + Rat brain lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 44 kDa
Anti-Gbeta5 antibody [EPR14778] (ab185206)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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