Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Gbeta5 antibody [EPR14778] (ab185206)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14778] to Gbeta5
  • Suitable for: WB, ICC/IF, IHC-P, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Gbeta5 antibody [EPR14778]
    See all Gbeta5 primary antibodies

  • Description

    Rabbit monoclonal [EPR14778] to Gbeta5

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Mouse
    Human
    IP
    Rat
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human cerebellum, rat and mouse brain lysates; Human lung adenocarcinoma and mouse brain tissues; HACAT and U87-MG cells.

  • General notes

     

     

    Previously labelled as GNB5.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR14778

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Western blot - Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Anti-Gbeta5 antibody [EPR14778] (ab185206) at 1/2000 dilution + Human cerebellum lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gbeta5 antibody [EPR14778] (ab185206)

    Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling Gbeta5 with ab185206 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Immunoprecipitation - Anti-Gbeta5 antibody [EPR14778] (ab185206)

    Western blot analysis of Gbeta5 in immunoprecipitation pellets from Rat brain lysate using ab185206 at 1/50 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.

  • Flow Cytometry - Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Flow Cytometry - Anti-Gbeta5 antibody [EPR14778] (ab185206)

    Flow cytometry analysis of 2% paraformaldehyde fixed HACAT cells labeling Gbeta5 with ab185206 at 1/160 dilution (red) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Immunocytochemistry/ Immunofluorescence - Anti-Gbeta5 antibody [EPR14778] (ab185206)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed U87-MG cells labeling Gbeta5 with ab185206 at 1/100 dilution. Secondary antibody: Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution. Counterstained with Dapi blue.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gbeta5 antibody [EPR14778] (ab185206)

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling Gbeta5 with ab185206 at 1/250 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Western blot - Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Anti-Gbeta5 antibody [EPR14778] (ab185206) at 1/10000 dilution + Mouse brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa

  • Western blot - Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Western blot - Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Anti-Gbeta5 antibody [EPR14778] (ab185206) at 1/10000 dilution + Rat brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 44 kDa

  • Anti-Gbeta5 antibody [EPR14778] (ab185206)
    Anti-Gbeta5 antibody [EPR14778] (ab185206)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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