Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Orai3 antibody [EPR22575-17] - BSA and Azide free (ab255336)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22575-17] to Orai3 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Orai3 antibody [EPR22575-17] - BSA and Azide free
    See all Orai3 primary antibodies

  • Description

    Rabbit monoclonal [EPR22575-17] to Orai3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human kidney and testis tissue lysates; RAW 264.7, NIH/3T3, MCF7 and A549 whole cell lysates. IHC-P: Human breast cancer, lung cancer and colon cancer tissue; mouse colon tissue.

  • General notes

    Ab255336 is the carrier-free version of ab254260. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab255336 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22575-17

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] - BSA and Azide free (ab255336)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] - BSA and Azide free (ab255336)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling Orai3 with ab254260 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining in mouse colon (PMID:30123430 ) is observed. Counter stained with hematoxylin.


    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.


    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254260).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] - BSA and Azide free (ab255336)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] - BSA and Azide free (ab255336)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue (A) and its adjacent non-cancerous tissue (B) labeling Orai3 with ab254260 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong membranous staining in human colon cancer tissue (A) while weak staining in its adjacent noncancerous tissue (B) (PMID:30123430 ) is observed. Counter stained with hematoxylin.


    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.


    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254260).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] - BSA and Azide free (ab255336)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] - BSA and Azide free (ab255336)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue (A) and its adjacent non-cancerous tissue (B) labeling Orai3 with ab254260 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong membranous staining in human lung cancer tissue (A) while weak staining in its adjacent noncancerous tissue (B) (PMID:24058448 ) is observed. Counter stained with hematoxylin.


    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.


    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254260).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] - BSA and Azide free (ab255336)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orai3 antibody [EPR22575-17] - BSA and Azide free (ab255336)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue (A) and its adjacent non-cancerous tissue (B) labeling Orai3 with ab254260 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Strong membranous staining in human breast cancer tissue (A) while weak staining in the adjacent noncancerous tissue (B) (PMID: 23266555) is observed. Counter stained with hematoxylin.


    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.


    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254260).
  • Anti-Orai3 antibody [EPR22575-17] - BSA and Azide free (ab255336)
    Anti-Orai3 antibody [EPR22575-17] - BSA and Azide free (ab255336)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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