Anti-ENO3 antibody [EPR11366(B)] - BSA and Azide free (ab249337)
![Anti-ENO3 antibody [EPR11366(B)] - BSA and Azide free (ab249337)](https://www.abcam.com/ps/products/249/ab249337/Images/ab249337-402608-anti-eno3-antibody-epr11366-b-bsa-and-azide-free-western-blot.jpg "Anti-ENO3 antibody [EPR11366(B)] - BSA and Azide free (ab249337)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11366(B)] to ENO3 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ENO3 antibody [EPR11366(B)] - BSA and Azide free
See all ENO3 primary antibodies -
Description
Rabbit monoclonal [EPR11366(B)] to ENO3 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab249337 is the carrier-free version of ab157474. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249337 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR11366(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ENO3 antibody [EPR11366(B)] - BSA and Azide free (ab249337)All lanes : Anti-ENO3 antibody [EPR11366(B)] (ab157474) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Human spleen lysate
Lane 4 : Fetal heart lysate
Lane 5 : Fetal muscle lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 47 kDaThis data was developed using ab157474, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-ENO3 antibody [EPR11366(B)] - BSA and Azide free (ab249337)](https://www.abcam.com/ps/products/249/ab249337/Images/ab249337-398099-anti-eno3-antibody-epr11366-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-ENO3 antibody [EPR11366(B)] - BSA and Azide free (ab249337)Anti-ENO3 antibody [EPR11366(B)] (ab157474) at 1/1000 dilution + immunoprecipitation pellet from fetal muscle lysate
Secondary
HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG
Predicted band size: 47 kDaThis data was developed using ab157474, the same antibody clone in a different buffer formulation.
Note: primary antibody diluted with 1% BSA.
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![Anti-ENO3 antibody [EPR11366(B)] - BSA and Azide free (ab249337)](https://www.abcam.com/ps/products/249/ab249337/Images/ab249337-3-benefits-of-recombinant-antibodies.png)
Anti-ENO3 antibody [EPR11366(B)] - BSA and Azide free (ab249337)
