Anti-SOX2 antibody (ab137385)
Key features and details
- Rabbit polyclonal to SOX2
- Suitable for: Flow Cyt, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-SOX2 antibody
See all SOX2 primary antibodies -
Description
Rabbit polyclonal to SOX2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF MouseHumanIHC-P HumanWB MouseHuman
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Immunogen
Recombinant fragment within Human SOX2 (internal sequence). The exact sequence is proprietary.
Database link: P48431 -
Positive control
- WB: Recombinant Human SOX2 protein (ab79950), NT2D1 whole cell lysate. Human and mouse embryonic stem cell lysate. ICC/IF: Human and mouse embryonic stem cells. A549 cells. IHC-P: Human Cal27 xenograft tissue. Flow Cytometry: Human embryonic stem cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SOX2 antibody (ab137385)Anti-SOX2 antibody (ab137385) at 1/1000 dilution + Human ESC whole cell lysate
at 20 µg
Predicted band size: 34 kDa10% SDS-PAGE gel
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (ab137385)Formalin-fixed, paraffin-embedded Cal27 xenograft tissue stained for SOX2 with ab137385 at a 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab137385)Human embryonic stem cells stained for SOX2 (green) using ab137385 (1/160 dilution) in ICC/IF. Lower panel is costained with Hoechst 33342.
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Flow Cytometry - Anti-SOX2 antibody (ab137385)Flow cytometric analysis of human embryonic stem cells, labelling SOX2 with ab137385 at 1/100 dilution (purple) compared to a rabbit IgG control (black).
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Western blot - Anti-SOX2 antibody (ab137385)Anti-SOX2 antibody (ab137385) at 1/1000 dilution + Mouse ESC whole cell lysate at 20 µg
Predicted band size: 34 kDa12% SDS-PAGE gel
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Western blot - Anti-SOX2 antibody (ab137385)Anti-SOX2 antibody (ab137385) at 1/3000 dilution + NT2D1 whole cell lysate
at 30 µg
Predicted band size: 34 kDa10% SDS-PAGE gel
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Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab137385)Mouse embryonic stem cells stained for SOX2 (green) using ab137385 (1/160 dilution) in ICC/IF. Lower panel is costained with Hoechst 33342.
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Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab137385)Paraformaldehyde-fixed A549 cells stained for SOX2 (green) using ab137385 (1/200 dilution) in ICC/IF. Lower panel is merged with a DNA probe.
