Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18114] to HMGA2 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-HMGA2 antibody [EPR18114] - BSA and Azide free
    See all HMGA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR18114] to HMGA2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251468 is the carrier-free version of ab207301. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251468 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
    Western blot - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
    All lanes : Anti-HMGA2 antibody [EPR18114] (ab207301) at 1/1000 dilution

    Lane 1 : HEK-293( Human embryonic kidney epithelial cell) 20 µg
    Lane 2 : HepG2 ( Human hepatocellular carcinoma epithelial cell)
    Lane 3 : NCCIT ( Human pluripotent embryonic carcinoma epithelial cell)

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 12 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab207301, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)

    This data was developed using ab207301, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling HMGA2 with ab207301 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human gastric cancer is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
    This data was developed using ab207301, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling HMGA2 with ab207301 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human colon cancer is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
    This data was developed using ab207301, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling HMGA2 with ab207301 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human colon tissue. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
    Immunocytochemistry - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
    This data was developed using ab207301, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling HMGA2 with ab207301 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).The negative controls are as follows:
    -ve control 1: ab207301 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
  • Immunocytochemistry - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
    Immunocytochemistry - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)

    This data was developed using ab207301, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling HMGA2 with ab207301 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HepG2 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).The negative controls are as follows:
    -ve control 1: ab207301 at 1/100 dilution followed by ab150120 (Alexa Fluor® 594 Goat anti-Mouse secondary) at 1/1000 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor® 488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

  • Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
    Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)

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