Anti-SOX2 antibody (ab239218)
Key features and details
- Goat polyclonal to SOX2
- Suitable for: Flow Cyt, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SOX2 antibody
See all SOX2 primary antibodies -
Description
Goat polyclonal to SOX2 -
Host species
Goat -
Tested applications
Suitable for: Flow Cyt, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Sheep, Chicken
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Immunogen
Synthetic peptide corresponding to Human SOX2 aa 1-100 (N terminal) (Cysteine residue). NP_003097.1
Database link: P48431 -
Positive control
- WB: MCF7 and U-251 MG nuclear cell lysate. ICC/IF: U-251 MG cells. Flow Cyt: MCF7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SOX2 antibody (ab239218)All lanes : Anti-SOX2 antibody (ab239218) at 0.3 µg/ml
Lane 1 : MCF7 (Human breast adenocarcinoma cell line) nuclear cell lysate in RIPA buffer
Lane 2 : U-251 MG (Human brain glioma cell line) nuclear cell lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.Detected by chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-SOX2 antibody (ab239218)Immunofluorescence analysis of paraformaldehyde fixed U-251 MG (Human brain glioma cell line) cells, permeabilized with 0.15% Triton, labeling SOX2 (green) using ab239218. Primary incubation time 1 hour (10 µg/ml) followed by an Alexa-Fluor®488 secondary antibody (2 µg/ml). Actin filaments were stained with phalloidin (red).
Negative control: Non-immunized goat IgG (10 µg/ml) followed by an Alexa-Fluor®488 secondary antibody (2 µg/ml).
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Flow Cytometry - Anti-SOX2 antibody (ab239218)Flow cytometric analysis of paraformaldehyde fixed MCF7 (Human breast adenocarcinoma cell line) cells (blue line), permeabilized with 0.5% Triton, labeling SOX2 (blue line) using ab239218. Primary incubation time 1 hour (10 µg/ml) followed by an Alexa-Fluor®488 secondary antibody (1 µg/ml).
IgG control: Non-immunized goat IgG (black line) followed by an Alexa-Fluor®488 secondary antibody.
