Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SOX2 (GlcNAc S248) antibody (ab136167)

Key features and details

  • Rabbit polyclonal to SOX2 (GlcNAc S248)
  • Suitable for: WB
  • Reacts with: Mouse
  • Isotype: IgG

Overview

  • Product name

    Anti-SOX2 (GlcNAc S248) antibody
    See all SOX2 primary antibodies

  • Description

    Rabbit polyclonal to SOX2 (GlcNAc S248)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Mouse SOX2 aa 200-300 (glcnac S248) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab157602)

  • Positive control

    • This antibody gave a positive signal in E14Tg2A and IOUD2 whole cell lysates.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab136167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Mouse
Application Abreviews Notes
WB (2)
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
Notes
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).

Target

  • Function

    Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.

  • Involvement in disease

    Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.

  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.

  • Post-translational
    modifications

    Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • ANOP3 antibody
      • cb236 antibody
      • Delta EF2a antibody
      • lcc antibody
      • MCOPS3 antibody
      • MGC148683 antibody
      • MGC2413 antibody
      • RGD1565646 antibody
      • Sex determining region Y box 2 antibody
      • SOX 2 antibody
      • Sox2 antibody
      • SOX2_HUMAN antibody
      • SRY (sex determining region Y) box 2 antibody
      • SRY box containing gene 2 antibody
      • SRY related HMG box 2 antibody
      • SRY related HMG box gene 2 antibody
      • SRY-box 2 antibody
      • Transcription factor SOX 2 antibody
      • Transcription factor SOX-2 antibody
      • ysb antibody
      see all

    Images

    • Western blot - Anti-SOX2 (GlcNAc S248) antibody (ab136167)
      Western blot - Anti-SOX2 (GlcNAc S248) antibody (ab136167)
      All lanes : Anti-SOX2 (GlcNAc S248) antibody (ab136167) at 1 µg/ml

      Lane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
      Lane 2 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate
      Lane 3 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate with SOX2 (glcnac S248) peptide (ab157602) at 1 µg/ml
      Lane 4 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate with SOX2 (glcnac S248) peptide (ab157602) at 1 µg/ml
      Lane 5 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate with Mouse SOX2 peptide (ab157771) at 1 µg/ml
      Lane 6 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate with Mouse SOX2 peptide (ab157771) at 1 µg/ml

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 34 kDa
      Observed band size: 40 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 148 kDa, 24 kDa, 29 kDa, 62 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 1 minute


      This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab136167 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    References (1)

    Publishing research using ab136167? Please let us know so that we can cite the reference in this datasheet.

    ab136167 has been referenced in 1 publication.

    • Speakman CM  et al. Elevated O-GlcNAc levels activate epigenetically repressed genes and delay mouse ES cell differentiation without affecting naïve to primed cell transition. Stem Cells N/A:N/A (2014). WB ; Mouse . PubMed: 24898611

    Images

    • Western blot - Anti-SOX2 (GlcNAc S248) antibody (ab136167)
      Western blot - Anti-SOX2 (GlcNAc S248) antibody (ab136167)
      All lanes : Anti-SOX2 (GlcNAc S248) antibody (ab136167) at 1 µg/ml

      Lane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
      Lane 2 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate
      Lane 3 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate with SOX2 (glcnac S248) peptide (ab157602) at 1 µg/ml
      Lane 4 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate with SOX2 (glcnac S248) peptide (ab157602) at 1 µg/ml
      Lane 5 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate with Mouse SOX2 peptide (ab157771) at 1 µg/ml
      Lane 6 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate with Mouse SOX2 peptide (ab157771) at 1 µg/ml

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 34 kDa
      Observed band size: 40 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 148 kDa, 24 kDa, 29 kDa, 62 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 1 minute


      This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab136167 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

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