Antibodies, Proteins, Kits and Reagents for Life Science

281 433 ₸ Product size

100 µg 281 433 ₸

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Anti-SOX2 (GlcNAc S248) antibody (ab136167)

Key features and details

Rabbit polyclonal to SOX2 (GlcNAc S248)
Suitable for: WB
Reacts with: Mouse
Isotype: IgG

Product name

Anti-SOX2 (GlcNAc S248) antibody
See all SOX2 primary antibodies
Description

Rabbit polyclonal to SOX2 (GlcNAc S248)
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Mouse

See all applications and species data
Immunogen

Synthetic peptide corresponding to Mouse SOX2 aa 200-300 (glcnac S248) conjugated to keyhole limpet haemocyanin.
(Peptide available as ab157602)
Positive control
- This antibody gave a positive signal in E14Tg2A and IOUD2 whole cell lysates.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS

Batches of this product that have a concentration
Concentration information loading...
Purity

Immunogen affinity purified
Clonality

Polyclonal
Isotype

IgG
Research areas

Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Corresponding Unmodified Peptide
- Mouse SOX2 peptide (ab157771)
Immunizing Peptide (Blocking)
- SOX2 (glcnac S248) peptide (ab157602)
Isotype control
- Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab136167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application	Species
WB	Mouse

Application	Abreviews	Notes
WB	(2)	Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).

Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).

Function

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
Involvement in disease

Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
Sequence similarities

Contains 1 HMG box DNA-binding domain.
Post-translational
modifications

Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
Cellular localization

Nucleus.
Target information above from: UniProt accession P48431 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 20674 Mouse
- SwissProt: P48432 Mouse
- Unigene: 65396 Mouse
Alternative names
- ANOP3 antibody
- cb236 antibody
- Delta EF2a antibody
- lcc antibody
- MCOPS3 antibody
- MGC148683 antibody
- MGC2413 antibody
- RGD1565646 antibody
- Sex determining region Y box 2 antibody
- SOX 2 antibody
- Sox2 antibody
- SOX2_HUMAN antibody
- SRY (sex determining region Y) box 2 antibody
- SRY box containing gene 2 antibody
- SRY related HMG box 2 antibody
- SRY related HMG box gene 2 antibody
- SRY-box 2 antibody
- Transcription factor SOX 2 antibody
- Transcription factor SOX-2 antibody
- ysb antibody
see all

Western blot - Anti-SOX2 (GlcNAc S248) antibody (ab136167)

All lanes : Anti-SOX2 (GlcNAc S248) antibody (ab136167) at 1 µg/ml

Lane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 2 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 3 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate with SOX2 (glcnac S248) peptide (ab157602) at 1 µg/ml
Lane 4 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate with SOX2 (glcnac S248) peptide (ab157602) at 1 µg/ml
Lane 5 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate with Mouse SOX2 peptide (ab157771) at 1 µg/ml
Lane 6 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate with Mouse SOX2 peptide (ab157771) at 1 µg/ml

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 34 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?
Additional bands at: 148 kDa, 24 kDa, 29 kDa, 62 kDa. We are unsure as to the identity of these extra bands.

Exposure time: 1 minute

This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab136167 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

Western blot protocols

Click here to view the general protocols

Datasheet

Publishing research using ab136167? Please let us know so that we can cite the reference in this datasheet.

ab136167 has been referenced in 1 publication.

Speakman CM et al. Elevated O-GlcNAc levels activate epigenetically repressed genes and delay mouse ES cell differentiation without affecting naïve to primed cell transition. Stem Cells N/A:N/A (2014). WB ; Mouse . PubMed: 24898611

Western blot - Anti-SOX2 (GlcNAc S248) antibody (ab136167)

All lanes : Anti-SOX2 (GlcNAc S248) antibody (ab136167) at 1 µg/ml

Lane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 2 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 3 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate with SOX2 (glcnac S248) peptide (ab157602) at 1 µg/ml
Lane 4 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate with SOX2 (glcnac S248) peptide (ab157602) at 1 µg/ml
Lane 5 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate with Mouse SOX2 peptide (ab157771) at 1 µg/ml
Lane 6 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate with Mouse SOX2 peptide (ab157771) at 1 µg/ml

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 34 kDa
Observed band size: 40 kDa
why is the actual band size different from the predicted?
Additional bands at: 148 kDa, 24 kDa, 29 kDa, 62 kDa. We are unsure as to the identity of these extra bands.

Exposure time: 1 minute

This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab136167 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.