Anti-NLK antibody (ab26050)
Key features and details
- Rabbit polyclonal to NLK
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NLK antibody
See all NLK primary antibodies -
Description
Rabbit polyclonal to NLK -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Dog, Xenopus laevis
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human NLK.
Read Abcam's proprietary immunogen policy (Peptide available as ab26986.) -
Positive control
- This antibody gave a positive signal in the following tissues: Formalin Fixed Paraffin Embedded Mouse Lung Normal; Formalin Fixed Paraffin Embedded Mouse Brain Normal. This antibody gave a positive signal in the following cell types: U-87 MG.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab26050 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes ICC/IF Use a concentration of 1 µg/ml. IHC-P Use at an assay dependent concentration. Target
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Function
Role in cell fate determination, required for differentiation of bone marrow stromal cells. Acts downstream of MAP3K7 and HIPK2 to negatively regulate the canonical Wnt/beta-catenin signaling pathway and the phosphorylation and destruction of the MYB transcription factor. May suppress a wide range of transcription factors by phosphorylation of the coactivator, CREBBP (By similarity). Involved in TGFbeta-mediated mesoderm induction, acting downstream of MAP3K7/TAK1 to phosphorylate STAT3. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Post-translational
modificationsDually phosphorylated on Thr-303 and Tyr-305, which activates the enzyme. -
Cellular localization
Nucleus. Cytoplasm. Predominantly nuclear. A smaller fraction is cytoplasmic. - Information by UniProt
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Database links
- Entrez Gene: 51701 Human
- Entrez Gene: 18099 Mouse
- Entrez Gene: 497961 Rat
- Omim: 609476 Human
- SwissProt: Q9UBE8 Human
- SwissProt: O54949 Mouse
- SwissProt: D3ZSZ3 Rat
- Unigene: 208759 Human
see all -
Alternative names
- DKFZp761G1211 antibody
- FLJ21033 antibody
- LAK1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLK antibody (ab26050)This image is courtesy of Melissa R. Mazan, DVMAnti-NLK was used in standard Vector ABC protocol on paraffin embedded mouse lung with the following modifications: HIER with Vector antigen unmasking solution for 25 minutes at 98C. Used a 1:100 dilution in PBS/Triton-X 4C overnight.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLK antibody (ab26050)Adult mouse brain fixed in 10% neutral buffered formulin. Antigen retrieval was performed using Tris-EDTA pH 9.0. The tissue was incubated with ab26050 at a concentration of 2.5µg/ml for 16 hours at 4°C. Nuclear staining can be clearly seen.
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Immunocytochemistry/ Immunofluorescence - Anti-NLK antibody (ab26050)ICC/IF image of ab26050 stained U87-MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab26050 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (5)
ab26050 has been referenced in 5 publications.
- Song L et al. c-Kit modifies the inflammatory status of smooth muscle cells. PeerJ 5:e3418 (2017). WB ; Mouse . PubMed: 28626608
- Rydell-Törmänen K et al. Aberrant nonfibrotic parenchyma in idiopathic pulmonary fibrosis is correlated with decreased ß-catenin inhibition and increased Wnt5a/b interaction. Physiol Rep 4:N/A (2016). IHC-P ; Human . PubMed: 26997628
- Suwei D et al. NLK functions to maintain proliferation and stemness of NSCLC and is a target of metformin. J Hematol Oncol 8:120 (2015). Human . PubMed: 26503334
- Shaw-Hallgren G et al. Association of nuclear-localized Nemo-like kinase with heat-shock protein 27 inhibits apoptosis in human breast cancer cells. PLoS One 9:e96506 (2014). IHC . PubMed: 24816797
- Ju H et al. Polyglutamine disease toxicity is regulated by Nemo-like kinase in spinocerebellar ataxia type 1. J Neurosci 33:9328-36 (2013). PubMed: 23719801
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLK antibody (ab26050) This image is courtesy of Melissa R. Mazan, DVMAnti-NLK was used in standard Vector ABC protocol on paraffin embedded mouse lung with the following modifications: HIER with Vector antigen unmasking solution for 25 minutes at 98C. Used a 1:100 dilution in PBS/Triton-X 4C overnight.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NLK antibody (ab26050)
Adult mouse brain fixed in 10% neutral buffered formulin. Antigen retrieval was performed using Tris-EDTA pH 9.0. The tissue was incubated with ab26050 at a concentration of 2.5µg/ml for 16 hours at 4°C. Nuclear staining can be clearly seen.
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Immunocytochemistry/ Immunofluorescence - Anti-NLK antibody (ab26050)
ICC/IF image of ab26050 stained U87-MG cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab26050 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
