Anti-KA1 antibody (ab67404)
Key features and details
- Rabbit polyclonal to KA1
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-KA1 antibody
See all KA1 primary antibodies -
Description
Rabbit polyclonal to KA1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
A 14 amino acid peptide near the amino terminus of human KA1.
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Positive control
- Rat brain lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-KA1 antibody (ab67404)Lane 1 : Anti-KA1 antibody (ab67404) at 0.5 µg/ml
Lane 2 : Anti-KA1 antibody (ab67404) at 1 µg/ml
Lane 3 : Anti-KA1 antibody (ab67404) at 2 µg/ml
All lanes : rat brain lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 107 kDa
Observed band size: -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KA1 antibody (ab67404)ab67404 at 2.5µg/ml staining of KA1 in human brain tissue by IHC-P. -
Immunocytochemistry/ Immunofluorescence - Anti-KA1 antibody (ab67404)ICC/IF image of ab67404 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67404, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunocytochemistry/ Immunofluorescence - Anti-KA1 antibody (ab67404)Immunofluorescence of Grik4 in Human Brain cells using ab67404 at 20 ug/ml.
