Anti-Neuron specific beta III Tubulin antibody (ab229590)
Key features and details
- Rabbit polyclonal to Neuron specific beta III Tubulin
- Suitable for: ICC/IF, IP, IHC-P, WB, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Neuron specific beta III Tubulin antibody -
Description
Rabbit polyclonal to Neuron specific beta III Tubulin -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IP, IHC-P, WB, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Neuron specific beta III Tubulin (internal sequence). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: Q13509 -
Positive control
- WB: HeLa, U-87 MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts; Mouse and rat brain tissue extracts. IP: SK-N-SH whole cell extract. IHC-P: Rat brain tissue. IHC-Fr: E13.5 rat brain tissue. ICC/IF: DIV9 rat E18 primary cortical neurons.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Neuron specific beta III Tubulin antibody (ab229590)All lanes : Anti-Neuron specific beta III Tubulin antibody (ab229590) at 1/5000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Neuron specific beta III Tubulin Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
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Western blot - Anti-Neuron specific beta III Tubulin antibody (ab229590)All lanes : Anti-Neuron specific beta III Tubulin antibody (ab229590) at 1/5000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : Neuron specific beta III Tubulin knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.10% SDS-PAGE gel.
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Immunohistochemistry (Frozen sections) - Anti-Neuron specific beta III Tubulin antibody (ab229590)Frozen E13.5 rat brain tissue stained for Neuron specific beta III Tubulin (green) using ab229590 at 1/250 dilution in immunohistochemical analysis.
Nuclear counterstain: Fluoroshield with DAPI (blue).
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Immunocytochemistry/ Immunofluorescence - Anti-Neuron specific beta III Tubulin antibody (ab229590)4 % paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for Neuron specific beta III Tubulin (green) using ab229590 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Fluoroshield with DAPI (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Neuron specific beta III Tubulin antibody (ab229590)Paraffin-embedded rat brain tissue stained for Neuron specific beta III Tubulin using ab229590 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-Neuron specific beta III Tubulin antibody (ab229590)All lanes : Anti-Neuron specific beta III Tubulin antibody (ab229590) at 1/1000 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.10% SDS-PAGE gel.
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Western blot - Anti-Neuron specific beta III Tubulin antibody (ab229590)All lanes : Anti-Neuron specific beta III Tubulin antibody (ab229590) at 1/50000 dilution
Lane 1 : Mouse brain tissue extract
Lane 2 : Rat brain tissue extract
Lysates/proteins at 50 µg per lane.10% SDS-PAGE gel.
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Immunoprecipitation - Anti-Neuron specific beta III Tubulin antibody (ab229590)Neuron specific beta III Tubulin was immunoprecipitated from SK-N-SH (human neuroblastoma cell line) whole cell extract with 5 μg ab229590. Western blot was performed from the immunoprecipitate using ab229590. Anti-rabbit IgG was used as a secondary antibody.
Lane 1: SK-N-SH whole cell extract (Input).
Lane 2: Control IgG instead of ab229590 in SK-N-SH whole cell extract.
Lane 3: ab229590 IP in SK-N-SH whole cell extract.