Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-LI Cadherin antibody [EPR3996] (ab109190)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3996] to LI Cadherin
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-LI Cadherin antibody [EPR3996]
    See all LI Cadherin primary antibodies

  • Description

    Rabbit monoclonal [EPR3996] to LI Cadherin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human small intestine, Human colon cancer, and HT-29 cell lysates. Human LoVo, small intestine and colon lysates. IHC-P: Human colon and Human colonic adenocarcinoma tissues

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Dissociation constant (KD)

    KD = 2.90 x 10 -12 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR3996

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-LI Cadherin antibody [EPR3996] (ab109190)
    Western blot - Anti-LI Cadherin antibody [EPR3996] (ab109190)
    Lanes 1-3 : Anti-LI Cadherin antibody [EPR3996] (ab109190) at 1/1000 dilution (Purified)
    Lanes 4-5 : Anti-LI Cadherin antibody [EPR3996] (ab109190) at 1/1000 dilution

    Lane 1 : LoVo (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
    Lane 2 : Human small intestine lysate
    Lane 3 : Human colon lysate
    Lane 4 : Human spleen lysate
    Lane 5 : Human kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 92 kDa
    Observed band size: 120 kDa
    why is the actual band size different from the predicted?



    The expression profile and molecular weight observed are consistent with what has been described in the literature (PMID: 18552820).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling LI Cadherin with purified ab109190 at 1/300 dilution (0.48 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)

    Immunohistochemical staining of paraffin-embedded Human colon tissue using ab109190 at a dilution of 1/250.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-LI Cadherin antibody [EPR3996] (ab109190)
    Western blot - Anti-LI Cadherin antibody [EPR3996] (ab109190)
    All lanes : Anti-LI Cadherin antibody [EPR3996] (ab109190) at 1/1000 dilution

    Lane 1 : Human small intestine cell lysate
    Lane 2 : Human colon cancer cell lysate
    Lane 3 : HT-29 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 92 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)

    Immunohistochemical staining of paraffin-embedded Human colonic adenocarcinoma tissue using ab109190 at a dilution of 1/250.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)

    ab109190 showing negative staining in Skeletal muscle tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)

    ab109190 showing negative staining in Normal kidney tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)

    ab109190 showing negative staining in Normal tonsil tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)

    ab109190 showing positive staining in Normal colon tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LI Cadherin antibody [EPR3996] (ab109190)

    ab109190 showing negative staining in Normal brain tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-LI Cadherin antibody [EPR3996] (ab109190)
    OI-RD Scanning - Anti-LI Cadherin antibody [EPR3996] (ab109190)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-LI Cadherin antibody [EPR3996] (ab109190)
    Anti-LI Cadherin antibody [EPR3996] (ab109190)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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