Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Tuberin antibody [EPR22886-235] (ab255612)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22886-235] to Tuberin
Suitable for: WB, IP
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-Tuberin antibody [EPR22886-235]
See all Tuberin primary antibodies
Description

Rabbit monoclonal [EPR22886-235] to Tuberin
Host species

Rabbit
Tested Applications & Species

Application Species
IP
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HAP1 (WT), C2C12, RAW 264.7 and HeLa whole cell lysates; human testis tissue lysate; mouse brain tissue lysate. IP: HeLa whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22886-235
Isotype

IgG
Research areas

Western blot - Anti-Tuberin antibody [EPR22886-235] (ab255612)

All lanes : Anti-Tuberin antibody [EPR22886-235] (ab255612) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : Tuberin knockout HAP1 whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 4 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 5 : A549 (human lung carcinoma epithelial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 200 kDa
Observed band size: 200 kDa

The molecular weight observed is consistent with what has been described in the literature (PMID:30774414; 10335945).

ab255612 was shown to specifically react with Tuberin in wild-type HAP1 cells as signal was lost in Tuberin knockout cells. Wild-type and Tuberin knockout samples were subjected to SDS-PAGE. ab255612 and ab129002 (Recombinant Anti-Vinculin antibody [EPR8185]) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/20,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD^® ChemiDoc^™ MP instrument using the ECL technique.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposire times.

Lanes 1-3: 81 seconds; Lane 4: 3 minutes.
Western blot - Anti-Tuberin antibody [EPR22886-235] (ab255612)

All lanes : Anti-Tuberin antibody [EPR22886-235] (ab255612) at 1/1000 dilution

Lane 1 : Human testis tissue lysate
Lane 2 : C2C12 (mouse myoblasts myoblast), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 200 kDa
Observed band size: 200 kDa

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID:30774414; 10335945).

This blot was developed using a higher sensitivity ECL substrate.
Western blot - Anti-Tuberin antibody [EPR22886-235] (ab255612)

All lanes : Anti-Tuberin antibody [EPR22886-235] (ab255612) at 1/1000 dilution

Lane 1 : Mouse brain tissue lysate
Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 200 kDa
Observed band size: 200 kDa

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID:30774414; 10335945).
Immunoprecipitation - Anti-Tuberin antibody [EPR22886-235] (ab255612)

Tuberin was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg with ab255612 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255612. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg

Lane 2: ab255612 IP in HeLa whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab255612 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.
Anti-Tuberin antibody [EPR22886-235] (ab255612)

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