Anti-Cleaved Caspase-3 antibody [EPR21032] - BSA and Azide free (ab231289)
![Anti-Cleaved Caspase-3 antibody [EPR21032] - BSA and Azide free (ab231289)](https://www.abcam.com/ps/products/231/ab231289/Images/ab231289-300542-214430.jpg "Anti-Cleaved Caspase-3 antibody [EPR21032] - BSA and Azide free (ab231289)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21032] to Cleaved Caspase-3 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse
Overview
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Product name
Anti-Cleaved Caspase-3 antibody [EPR21032] - BSA and Azide free
See all Cleaved Caspase-3 primary antibodies -
Description
Rabbit monoclonal [EPR21032] to Cleaved Caspase-3 - BSA and Azide free -
Host species
Rabbit -
Specificity
ab214430 recognises both pro-Caspase-3 and p17 cleavage fragments. -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: NIH/3T3 cells treated with staurosporine, whole cell lysate.
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General notes
Ab231289 is the carrier-free version of ab214430. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab231289 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21032 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Cleaved Caspase-3 antibody [EPR21032] - BSA and Azide free (ab231289)All lanes : Anti-Cleaved Caspase-3 antibody [EPR21032] (ab214430) at 1/5000 dilution
Lane 1 : Untreated NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg
Lane 2 : NIH/3T3 cells treated with 1 uM staurosprine for 4 hours, whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 31 kDa
Observed band size: 14,19,24,29,32 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and dilution buffer: 5% NFDM/TBST.
Observed molecular masses are consistent with the literature for full-length and cleaved caspase 3 (PMID: 9922454, PMID 16221205).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab214430).
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![Anti-Cleaved Caspase-3 antibody [EPR21032] - BSA and Azide free (ab231289)](https://www.abcam.com/ps/products/231/ab231289/Images/ab231289-2-benefits-of-recombinant-antibodies.png)
Anti-Cleaved Caspase-3 antibody [EPR21032] - BSA and Azide free (ab231289)
