Anti-Cleaved Caspase-3 antibody [E83-77] - BSA and Azide free (ab208003)
![Anti-Cleaved Caspase-3 antibody [E83-77] - BSA and Azide free (ab208003)](https://www.abcam.com/ps/products/208/ab208003/Images/ab208003-329274-anti-active-caspase-3-antibody-e83-77-immunofluorescence.jpg "Anti-Cleaved Caspase-3 antibody [E83-77] - BSA and Azide free (ab208003)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E83-77] to Cleaved Caspase-3 - BSA and Azide free
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Cleaved Caspase-3 antibody [E83-77] - BSA and Azide free
See all Cleaved Caspase-3 primary antibodies -
Description
Rabbit monoclonal [E83-77] to Cleaved Caspase-3 - BSA and Azide free -
Host species
Rabbit -
Specificity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This antibody only detects the active (cleaved) form of Caspase-3 and does not recognize the pro form of Caspase-3. PubMedID 19789217 describes the detection of human cells injected into mice. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Jurkat cell lysate. IF: Hela cells.
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General notes
Ab208003 is the carrier-free version of ab32042. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab208003 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E83-77 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Cleaved Caspase-3 antibody [E83-77] - BSA and Azide free (ab208003)Ab32042, at dilution of 1/100, staining HeLa cells by Immunofluorescence.
Left image: control.
Right image: staurosporine treated.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32042).
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![Anti-Cleaved Caspase-3 antibody [E83-77] - BSA and Azide free (ab208003)](https://www.abcam.com/ps/products/208/ab208003/Images/ab208003-2-benefits-of-recombinant-antibodies.png)
Anti-Cleaved Caspase-3 antibody [E83-77] - BSA and Azide free (ab208003)
