Recombinant Envelope Glycoprotein (His tag) (ab230280)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Tags: His tag C-Terminus
- Suitable for: WB, SDS-PAGE, ELISA
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Product name
Recombinant Envelope Glycoprotein (His tag)
See all Envelope Glycoprotein proteins and peptides -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Ebolavirus -
Amino acids
33 to 676 -
Tags
His tag C-Terminus -
Additional sequence information
Minus the transmembrane domain.
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Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
Constituent: PBS
Supplemented with glycerol, arginine and glutamic acid
Images
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SDS-PAGE - Recombinant Envelope Glycoprotein (His tag) (ab230280)
SDS-PAGE analysis of 5 µg and 1 µg (lane 1, 2 respectively) ab230280.
Because of the highly glycosylated nature of this protein, migration in an SDS-PAGE gel is slowed resulting in broad, diffuse bands representing differing glycosylation forms.
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Western blot - Recombinant Envelope Glycoprotein (His tag) (ab230280)All lanes : Rabbit anti-EBOV Envelope Glycoprotein polyclonal antibody
Lane 1 :Recombinant Envelope Glycoprotein (His tag) (ab230280) at 0.05 µg
Lane 2 :Recombinant Envelope Glycoprotein (His tag) (ab230280) at 0.1 µg
Lane 3 :Recombinant Envelope Glycoprotein (His tag) (ab230280) at 0.5 µg
Secondary
All lanes : Anti-rabbit IgG-HRP conjugateFollowed by TMB membrane substrate.
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ELISA - Recombinant Envelope Glycoprotein (His tag) (ab230280)ELISA analysis of ab230280. Plate was coated with ab230280 starting at 800 ng/well, serially diluted in DPBS. Washed plate was detected using one dilution of a positive control serum, followed with anti-IgG HRP conjugate and TMB substrate. OD650 is reported. Background of ab230280 coated plate positive control serum was 0.071 OD650.
