Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant Envelope Glycoprotein (His tag) (ab220456)

Key features and details

  • Expression system: HEK 293 cells
  • Purity: > 85% SDS-PAGE
  • Endotoxin level:
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE

Description

  • Product name

    Recombinant Envelope Glycoprotein (His tag)
    See all Envelope Glycoprotein proteins and peptides

  • Purity

    > 85 % SDS-PAGE.

  • Endotoxin level

  • Expression system

    HEK 293 cells

  • Accession

    P87666

  • Protein length

    Protein fragment

  • Animal free

    No

  • Nature

    Recombinant

    • Sequence

      IPLGVIHNSTLQVSDVDKLVCRDKLSSTNQLRSVGLNLEGNGVATDVPSA TKRWGFRSGVPPKVVNYEAGEWAENCYNLEIKKPDGSECLPAAPDGIRGF PRCRYVHKVSGTGPCAGDFAFHKEGAFFLYDRLASTVIYRGTTFAEGVVA FLILPQAKKDFFSSHPLREPVNATEDPSSGYYSTTIRYQATGFGTNETEY LFEVDNLTYVQLESRFTPQFLLQLNETIYTSGKRSNTTGKLIWKVNPEID TTIGEWAFWETKKNLTRKIRSEELSFTAVSNRAKNISGQSPARTSSDPGT NTTTEDHKIMASENSSAMVQVHSQGREAAVSHLTTLATISTSPQPPTTKP GPDNSTHNTPVYKLDISEATQVEQHHRRTDNDSTASDTPPATTAAGPLKA ENTNTSKGTDLLDPATTTSPQNHSETAGNNNTHHQDTGEESASSGKLGLI TNTIAGVAGLITGGRRARREAIVNAQPKCNPNLHYWTTQDEGAAIGLAWI PYFGPAAEGIYTEGLMHNQDGLICGLRQLANETTQALQLFLRATTELRTF SILNRKAIDFLLQRWGGTCHILGPDCCIEPHDWTKNITDKIDQIIHDFVD KTLPD

    • Predicted molecular weight

      67 kDa including tags

    • Amino acids

      33 to 637

    • Tags

      His tag C-Terminus

    • Additional sequence information

      Ebolavirus EBOV (subtype Zaire,strain Kikwit-95)

    • Specifications

    Our Abpromise guarantee covers the use of ab220456 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Lyophilized
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.

      pH: 7.40
      Constituents: 5% Trehalose, 95% PBS

      Lyophilized from 0.22 µm filtered solution.
      5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%

    • Reconstitution
      Reconstitute with sterile deionized water to a concentration of 200 µg/ml.

    General Info

    • Alternative names

      • GP
      • GP 1,2
      • GP1
      • GP2
      • GP2-delta
      • Spike glycoprotein
      • Virion spike glycoprotein
      see all

    • Cellular localization

      Virion membrane; Peripheral membrane protein.

Images

  • SDS-PAGE - Recombinant Envelope Glycoprotein (His tag) (ab220456)
    SDS-PAGE - Recombinant Envelope Glycoprotein (His tag) (ab220456)

    SDS-PAGE using ab220456. DTT-reduced protein migrates as 21-23 kDa (GP2-delta) and 110-120 kDa (GP1) in SDS-PAGE .

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab220456? Please let us know so that we can cite the reference in this datasheet.

ab220456 has not yet been referenced specifically in any publications.

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: 5% Trehalose, 95% PBS

    Lyophilized from 0.22 µm filtered solution.
    5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%

  • Reconstitution
    Reconstitute with sterile deionized water to a concentration of 200 µg/ml.

Images

  • SDS-PAGE - Recombinant Envelope Glycoprotein (His tag) (ab220456)
    SDS-PAGE - Recombinant Envelope Glycoprotein (His tag) (ab220456)

    SDS-PAGE using ab220456. DTT-reduced protein migrates as 21-23 kDa (GP2-delta) and 110-120 kDa (GP1) in SDS-PAGE .

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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