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Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6392] to Werner's syndrome helicase WRN
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Werner's syndrome helicase WRN antibody [EPR6392]
    See all Werner's syndrome helicase WRN primary antibodies

  • Description

    Rabbit monoclonal [EPR6392] to Werner's syndrome helicase WRN

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Werner's syndrome helicase WRN aa 1400-1500 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HAP1, MOLT 4, K562 and A431 cell lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR6392

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673)
    Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673)
    All lanes : Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : WRN knockout HAP1 cell lysate
    Lane 3 : MOLT-4 cell lysate
    Lane 4 : K562 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 162 kDa
    Observed band size: 170 kDa
    why is the actual band size different from the predicted?



    Lanes 1 - 4: Merged signal (red and green). Green - ab124673 observed at 170 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

    ab124673 was shown to react with Werner's syndrome helicase WRN in wild-type HAP1 cells in western blot. Loss of signal was observed when WRN knockout sample was used. Wild-type and WRN knockout HAP1 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab124673 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673)
    Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Werner's syndrome helicase WRN knockout HAP1 cell lysate (20 µg)
    Lane 3: MOLT4 cell lysate (20 µg)
    Lane 4: K562 cell lysate (20 µg)
    Lanes 1 to 4: Merged signal (red and green). Green - ab124673 observed at 170 kDa. Red - loading control, ab181602, observed at 124 kDa.
    ab124673 was shown to recognize Werner's syndrome helicase WRN when Werner's syndrome helicase WRN knockout samples were used, along with additional cross-reactive bands. Wild-type and Werner's syndrome helicase WRN knockout samples were subjected to SDS-PAGE. ab124673 and ab181602 (loading control to GAPDH) were both diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673)
    Western blot - Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673)
    All lanes : Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673) at 1/1000 dilution

    Lane 1 : MOLT4 cell lysates
    Lane 2 : K562 cell lysates
    Lane 3 : A431 cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 162 kDa

  • Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673)
    Anti-Werner's syndrome helicase WRN antibody [EPR6392] (ab124673)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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