Anti-Werner's syndrome helicase WRN antibody (ab200)
Key features and details
- Rabbit polyclonal to Werner's syndrome helicase WRN
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Werner's syndrome helicase WRN antibody
See all Werner's syndrome helicase WRN primary antibodies -
Description
Rabbit polyclonal to Werner's syndrome helicase WRN -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide, corresponding to amino acids 1-510 of Human Werner's syndrome helicase WRN
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Werner's syndrome helicase WRN antibody (ab200)ab200 (4µg/ml) staining Werner's syndrome helicase WRN in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is nuclear and some cytoplasmic staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
