Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)
![Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)](https://www.abcam.com/ps/products/256/ab256799/Images/ab256799-448943-anti-ror-alpharora-antibody-epr2371918-western-blot-lysate.jpg "Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23719-18] to ROR alpha/RORA
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Rat
Overview
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Product name
Anti-ROR alpha/RORA antibody [EPR23719-18]
See all ROR alpha/RORA primary antibodies -
Description
Rabbit monoclonal [EPR23719-18] to ROR alpha/RORA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseRatIP MouseRatWB MouseRat
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse and rat cerebellum tissue lysate. IHC-P: Mouse and rat cerebellum tissue. IP: Mouse and rat cerebellum tissue lysate.
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General notes
This product is produced using clone 18 of EPR23719.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23719-18 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)All lanes : Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799) at 1/1000 dilution
Lane 1 : Mouse cerebellum tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 60, 65 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: mouse spleen (PMID:9226375).
The molecular weight observed is consistent with what has been described in the literature (PMID:19955433, PMID:12023888)
Exposure time: 3 minutes
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)](https://www.abcam.com/ps/products/256/ab256799/Images/ab256799-1-anti-ror-alpharora-antibody-epr2371918-immunohistochemistry-mouse-cerebellum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling ROR alpha/RORA with ab256799 at 1/800 (0.601 µg/ml) dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse cerebellum (PMID:14966198). The section was incubated with ab256799 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Immunoprecipitation - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)](https://www.abcam.com/ps/products/256/ab256799/Images/ab256799-3-anti-ror-alpharora-antibody-epr2371918-immunoprecipitation-mouse-cerebellum-tissue-lysate-10ug.jpg)
Immunoprecipitation - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)ROR alpha/RORA was immunoprecipitated from 0.35 mg mouse cerebellum tissue lysate with ab256799 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256799 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse cerebellum tissue lysate 10µg
Lane 2: ab256799 IP in mouse cerebellum tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256799 in mouse cerebellum tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
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![Western blot - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)](https://www.abcam.com/ps/products/256/ab256799/Images/ab256799-448945-anti-ror-alpharora-antibody-epr2371918-western-blot-lysate1.jpg)
Western blot - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799) at 1/1000 dilution + Rat cerebellum tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 63 kDa
Observed band size: 60, 65 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 minutes
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)](https://www.abcam.com/ps/products/256/ab256799/Images/ab256799-2-anti-ror-alpharora-antibody-epr2371918-immunohistochemistry-rat-cerebellum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling ROR alpha/RORA with ab256799 at 1/800 (0.601 µg/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat cerebellum. The section was incubated with ab256799 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunoprecipitation - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)](https://www.abcam.com/ps/products/256/ab256799/Images/ab256799-4-anti-ror-alpharora-antibody-epr2371918-immunoprecipitation-rat-cerebellum-tissue-lysate-10ug.jpg)
Immunoprecipitation - Anti-ROR alpha/RORA antibody [EPR23719-18] (ab256799)ROR alpha/RORA was immunoprecipitated from 0.35 mg rat cerebellum tissue lysate with ab256799 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256799 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat cerebellum tissue lysate 10µg
Lane 2: ab256799 IP in rat cerebellum tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256799 in rat cerebellum tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.