Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - BSA and Azide free (ab209972)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15656] to Androgen Receptor (AR-V7 specific) - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - BSA and Azide free
    See all Androgen Receptor (AR-V7 specific) primary antibodies

  • Description

    Rabbit monoclonal [EPR15656] to Androgen Receptor (AR-V7 specific) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 22Rv1 whole cell lysate. IHC-P: Human prostate cancer and breast tumor tissue.

  • General notes

    ab209972 is the carrier-free version of abyyyyy. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab209972 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Note that for IHC it is recommended to use high pH (9.0) antigen retrieval buffer and a high sensitivity detection system, such as our Rabbit Specific IHC polymer detection kit HRP/DAB (ab209101). 

    For Androgen receptor variant 5,6,7es (ARv567es) specific RabMAb product - see ab200827.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15656

  • Isotype

    IgG

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - BSA and Azide free (ab209972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - BSA and Azide free (ab209972)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

    Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Nucleus staining on cancer cells of human prostate cancer (case#:F123132) is observed.

    Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

    Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

    PLoS One. 2011;6(11):e27970. PMID:22114732

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198394).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - BSA and Azide free (ab209972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - BSA and Azide free (ab209972)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling AR-V7 with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

    Negative staining on human hepatocellular carcinoma.

    Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

    Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198394).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - BSA and Azide free (ab209972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - BSA and Azide free (ab209972)

    Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

    Negative staining on normal human liver tissue.

    Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

    Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198394).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - BSA and Azide free (ab209972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - BSA and Azide free (ab209972)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

    Negative on cancer cells of human prostate cancer (case#:F121524)

    Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

    Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

    PLoS One. 2011;6(11):e27970. PMID:22114732

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198394).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - BSA and Azide free (ab209972)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - BSA and Azide free (ab209972)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue labeling Androgen Receptor(AR-V7) with ab198394 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Counterstained with hematoxylin.

    Negative on cancer cells of human prostate cancer (case#:F11194)

    Cancer Res. 2014 Apr 15;74(8):2270-82. PMID:24556717

    Cancer Res. 2009 Jan 1;69(1):16-22. PMID:19117982

    PLoS One. 2011;6(11):e27970. PMID:22114732

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab198394).

  • Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - BSA and Azide free (ab209972)
    Anti-Androgen Receptor (AR-V7 specific) antibody [EPR15656] - BSA and Azide free (ab209972)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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