Anti-ABHD12 antibody [EPR13683] - BSA and Azide free (ab250276)
![Anti-ABHD12 antibody [EPR13683] - BSA and Azide free (ab250276)](https://www.abcam.com/ps/products/250/ab250276/Images/ab250276-403481-anti-abhd12-antibody-epr13683-bsa-and-azide-free-western-blot.jpg "Anti-ABHD12 antibody [EPR13683] - BSA and Azide free (ab250276)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13683] to ABHD12 - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-ABHD12 antibody [EPR13683] - BSA and Azide free
See all ABHD12 primary antibodies -
Description
Rabbit monoclonal [EPR13683] to ABHD12 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250276 is the carrier-free version of ab180944. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250276 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR13683 -
Isotype
IgG
Images
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Western blot - Anti-ABHD12 antibody [EPR13683] - BSA and Azide free (ab250276)All lanes : Anti-ABHD12 antibody [EPR13683] (ab180944) at 1/5000 dilution
Lane 1 : SH-SY5Y lysate
Lane 2 : U87-MG lysate
Lane 3 : THP-1 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDaThis data was developed using ab180944, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Flow Cytometry - Anti-ABHD12 antibody [EPR13683] - BSA and Azide free (ab250276)](https://www.abcam.com/ps/products/250/ab250276/Images/ab250276-1-anti-abhd12-antibody-epr13683-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-ABHD12 antibody [EPR13683] - BSA and Azide free (ab250276)This data was developed using ab180944, the same antibody clone in a different buffer formulation.Flow Cytometry analysis of SH-SY5Y cells labeling ABHD12 using ab180944 at a 1/170 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde. -
![Anti-ABHD12 antibody [EPR13683] - BSA and Azide free (ab250276)](https://www.abcam.com/ps/products/250/ab250276/Images/ab250276-3-benefits-of-recombinant-antibodies.png)
Anti-ABHD12 antibody [EPR13683] - BSA and Azide free (ab250276)