Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8283(B)] to Aconitase 2
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Aconitase 2 antibody [EPR8283(B)]
    See all Aconitase 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR8283(B)] to Aconitase 2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Aconitase 2 aa 500-600. The exact sequence is proprietary.

  • Positive control

    • HeLa, K562, Jurkat and 293T cell lysates; Human kidney tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
    Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
    All lanes : Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105) at 1/1000 dilution

    All lanes : Wild-type HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 85 kDa
    Observed band size: 85 kDa



    Lanes 1 - 2: Merged signal (red and green). Green - ab129105 observed at 85 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab129105 was shown to specifically react with Aconitase 2 in wild-type HAP1 cells as signal was lost in Hela knockout cells. Wild-type and Hela knockout samples were subjected to SDS-PAGE. Ab129105 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
    Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
    All lanes : Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 85 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)

    ab129105, at 1/100 dilution, staining Aconitase 2 in Paraffin-embedded Human kidney tissue by immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
    Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105) This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
    All lanes : Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105) at 1/1000 dilution

    Lane 1 : 1 month old Mouse Liver Mitochondrial fraction
    Lanes 2-3 : 3 month old Mouse Liver Mitochondrial fraction
    Lane 4 : 6 month old Mouse Liver Mitochondrial fraction

    Secondary
    All lanes : Pig Polyclonal to anti-Rabbit (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 85 kDa


    Exposure time: 1 minute


    Blocking: 5% milk for 1 hour at 24°C

    See Abreview

  • OI-RD Scanning - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
    OI-RD Scanning - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)
    Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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