Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8283(B)] to Aconitase 2
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-Aconitase 2 antibody [EPR8283(B)]
See all Aconitase 2 primary antibodies
Description

Rabbit monoclonal [EPR8283(B)] to Aconitase 2
Host species

Rabbit
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human Aconitase 2 aa 500-600. The exact sequence is proprietary.
Positive control
- HeLa, K562, Jurkat and 293T cell lysates; Human kidney tissue.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 2.60 x 10 ^-10 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8283(B)
Isotype

IgG
Research areas

Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)

All lanes : Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105) at 1/1000 dilution

All lanes : Wild-type HAP1 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 85 kDa
Observed band size: 85 kDa

Lanes 1 - 2: Merged signal (red and green). Green - ab129105 observed at 85 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab129105 was shown to specifically react with Aconitase 2 in wild-type HAP1 cells as signal was lost in Hela knockout cells. Wild-type and Hela knockout samples were subjected to SDS-PAGE. Ab129105 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)

All lanes : Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 85 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)

ab129105, at 1/100 dilution, staining Aconitase 2 in Paraffin-embedded Human kidney tissue by immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105) This image is courtesy of an abreview submitted by Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

All lanes : Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105) at 1/1000 dilution

Lane 1 : 1 month old Mouse Liver Mitochondrial fraction
Lanes 2-3 : 3 month old Mouse Liver Mitochondrial fraction
Lane 4 : 6 month old Mouse Liver Mitochondrial fraction

Secondary
All lanes : Pig Polyclonal to anti-Rabbit (HRP) at 1/3000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 85 kDa

Exposure time: 1 minute

Blocking: 5% milk for 1 hour at 24°C
See Abreview
OI-RD Scanning - Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-Aconitase 2 antibody [EPR8283(B)] (ab129105)

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