Anti-Aconitase 2 antibody [6D1BE4] (ab110320)
![Anti-Aconitase 2 antibody [6D1BE4] (ab110320)](https://www.abcam.com/ps/products/110/ab110320/Images/Aconitase-2-Primary-antibodies-ab110320-4.gif "Anti-Aconitase 2 antibody [6D1BE4] (ab110320)")
Key features and details
- Mouse monoclonal [6D1BE4] to Aconitase 2
- Suitable for: IP, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-Aconitase 2 antibody [6D1BE4]
See all Aconitase 2 primary antibodies -
Description
Mouse monoclonal [6D1BE4] to Aconitase 2 -
Host species
Mouse -
Specificity
This antibody is not suitable for Western blot. We recommend ab110321 for Western blotting. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIP MouseRatHuman
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Immunogen
This information is considered to be commercially sensitive.
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Positive control
- HDFn cells and HeLa cells; Hela, HepG2, Rat and Mouse liver cell lysates.
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purification notes
ab110320 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity: >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
6D1BE4 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Aconitase 2 antibody [6D1BE4] (ab110320)ab110320 at 1µg/ml staining Aconitase 2 in HDFn cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red).
Note: the target protein locates to the mitochondria. -
![Immunoprecipitation - Anti-Aconitase 2 antibody [6D1BE4] (ab110320)](https://www.abcam.com/ps/products/110/ab110320/Images/ab110320-219533-Comassie-blue-image.jpg)
Immunoprecipitation - Anti-Aconitase 2 antibody [6D1BE4] (ab110320)Detection of ab110321 by colloidal Coomassie blue G staining of Immunprecipitate.
Staining of Aconitase in HeLa, HepG2, Rat liver homogenate (RLH), and Mouse liver homogenate (MLH) lysates immunoprecipitated using ab110320. -
![Flow Cytometry - Anti-Aconitase 2 antibody [6D1BE4] (ab110320)](https://www.abcam.com/ps/products/110/ab110320/Images/Aconitase-2-Primary-antibodies-ab110320-3.jpg)
Flow Cytometry - Anti-Aconitase 2 antibody [6D1BE4] (ab110320)ab110320 at 1µg/ml staining Aconitase 2 in HeLa cells by Flow Cytometry (blue). Isotype control antibody (red).
